Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22889 | 3' | -56.6 | NC_005137.2 | + | 67918 | 0.7 | 0.612746 |
Target: 5'- cGGGCAGAUUGUuaaaCACUacGUCGCCGGGu -3' miRNA: 3'- -CCCGUCUGGCGc---GUGAa-UAGCGGCUU- -5' |
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22889 | 3' | -56.6 | NC_005137.2 | + | 88491 | 0.71 | 0.58181 |
Target: 5'- cGGGCGGGCgagcgauuaGCGCGCUaUAcCGCCGGu -3' miRNA: 3'- -CCCGUCUGg--------CGCGUGA-AUaGCGGCUu -5' |
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22889 | 3' | -56.6 | NC_005137.2 | + | 22845 | 0.72 | 0.52111 |
Target: 5'- aGGGCGGGCgCGCGCAUaccgaCGCCGc- -3' miRNA: 3'- -CCCGUCUG-GCGCGUGaaua-GCGGCuu -5' |
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22889 | 3' | -56.6 | NC_005137.2 | + | 102925 | 0.72 | 0.482034 |
Target: 5'- cGGCAGGCCGCGCuGCgUGUacaGCCGu- -3' miRNA: 3'- cCCGUCUGGCGCG-UGaAUAg--CGGCuu -5' |
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22889 | 3' | -56.6 | NC_005137.2 | + | 13705 | 0.76 | 0.304507 |
Target: 5'- uGGGCGcGACCGCGUugUUGcuauuuuUUGCCGAc -3' miRNA: 3'- -CCCGU-CUGGCGCGugAAU-------AGCGGCUu -5' |
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22889 | 3' | -56.6 | NC_005137.2 | + | 52274 | 1.09 | 0.002102 |
Target: 5'- cGGGCAGACCGCGCACUUAUCGCCGAAg -3' miRNA: 3'- -CCCGUCUGGCGCGUGAAUAGCGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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