Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22893 | 3' | -59.8 | NC_005137.2 | + | 63280 | 0.66 | 0.710421 |
Target: 5'- cUCCaacaCggUCuCGcCGUCGCUCGCGGGCa -3' miRNA: 3'- -AGGc---GuuGGuGC-GCAGCGGGUGCCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 48235 | 0.66 | 0.720168 |
Target: 5'- uUCCGUugcuGCCGCcguuacCGUCGCCCACa--- -3' miRNA: 3'- -AGGCGu---UGGUGc-----GCAGCGGGUGccug -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 100257 | 0.66 | 0.720168 |
Target: 5'- gCCGCAACgCACGgGcgCGCgUCGCGG-Ca -3' miRNA: 3'- aGGCGUUG-GUGCgCa-GCG-GGUGCCuG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 10724 | 0.66 | 0.739435 |
Target: 5'- aCCGCAucGCgCACGCGUCGgCCAg---- -3' miRNA: 3'- aGGCGU--UG-GUGCGCAGCgGGUgccug -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 42241 | 0.66 | 0.748938 |
Target: 5'- -gCGCGAgCACGCGUUGCCUcucaacuacaACGaGCu -3' miRNA: 3'- agGCGUUgGUGCGCAGCGGG----------UGCcUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 53277 | 0.66 | 0.720168 |
Target: 5'- aCUGCAugCAgGUGUuuaaCGCCCACGa-- -3' miRNA: 3'- aGGCGUugGUgCGCA----GCGGGUGCcug -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 52069 | 0.66 | 0.729842 |
Target: 5'- -aCGCGAa-ACGCGUUGUCgCACGGuACa -3' miRNA: 3'- agGCGUUggUGCGCAGCGG-GUGCC-UG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 12443 | 0.66 | 0.710421 |
Target: 5'- gUCCGUGAUCAgGCGcuuucaUGUCCACGGuCg -3' miRNA: 3'- -AGGCGUUGGUgCGCa-----GCGGGUGCCuG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 96412 | 0.67 | 0.640881 |
Target: 5'- aCCGCuacCCGCGCGgcgacgCGCCCGCc--- -3' miRNA: 3'- aGGCGuu-GGUGCGCa-----GCGGGUGccug -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 35167 | 0.67 | 0.660901 |
Target: 5'- --gGCAACaauC-CGUCGCCCGCuGGGCa -3' miRNA: 3'- aggCGUUGgu-GcGCAGCGGGUG-CCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 102960 | 0.67 | 0.660901 |
Target: 5'- aUCC-CAG-CGCGCGUCGCgCCACGuuCg -3' miRNA: 3'- -AGGcGUUgGUGCGCAGCG-GGUGCcuG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 98118 | 0.67 | 0.680833 |
Target: 5'- aCCGCcGCCGgcuacCGCGUCGUCguacgcgcgUugGGACu -3' miRNA: 3'- aGGCGuUGGU-----GCGCAGCGG---------GugCCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 88794 | 0.67 | 0.660901 |
Target: 5'- gCCGUuGCCacucguuguagcGCGCGUCGCgUcccugucgGCGGACg -3' miRNA: 3'- aGGCGuUGG------------UGCGCAGCGgG--------UGCCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 39818 | 0.67 | 0.670882 |
Target: 5'- -gUGCAACCAaaCGcCGCgCCACGGAa -3' miRNA: 3'- agGCGUUGGUgcGCaGCG-GGUGCCUg -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 130864 | 0.67 | 0.670882 |
Target: 5'- -gCGCAGCC-CGCGccugaaUUGUacgCCGCGGACg -3' miRNA: 3'- agGCGUUGGuGCGC------AGCG---GGUGCCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 110808 | 0.67 | 0.670882 |
Target: 5'- uUUCGCAAUCgACGC--CGCCCGCcGACg -3' miRNA: 3'- -AGGCGUUGG-UGCGcaGCGGGUGcCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 18257 | 0.67 | 0.680833 |
Target: 5'- aCUGCGGCgC-CGCGUcCGUuaaCACGGACa -3' miRNA: 3'- aGGCGUUG-GuGCGCA-GCGg--GUGCCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 38085 | 0.67 | 0.680833 |
Target: 5'- aCUGaCcGCCACGgacaaGCCCACGGGCa -3' miRNA: 3'- aGGC-GuUGGUGCgcag-CGGGUGCCUG- -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 110454 | 0.67 | 0.680833 |
Target: 5'- aCCGCGGCCgGCGCGUa---CACGGGg -3' miRNA: 3'- aGGCGUUGG-UGCGCAgcggGUGCCUg -5' |
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22893 | 3' | -59.8 | NC_005137.2 | + | 30977 | 0.67 | 0.690745 |
Target: 5'- gCCGCGucggACCGCGUGcuugCGCaCCGCuGGAg -3' miRNA: 3'- aGGCGU----UGGUGCGCa---GCG-GGUG-CCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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