Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22899 | 5' | -45.6 | NC_005137.2 | + | 32864 | 1.11 | 0.019886 |
Target: 5'- gCUAUAAAAGGCGCGGCCGAAUAAACAa -3' miRNA: 3'- -GAUAUUUUCCGCGCCGGCUUAUUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 119799 | 0.69 | 0.999018 |
Target: 5'- -------cGGCGaCGGCCGGAgcGGCGc -3' miRNA: 3'- gauauuuuCCGC-GCCGGCUUauUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 95600 | 0.69 | 0.999018 |
Target: 5'- -aAUGAuGGGCGCGcGCCGAu------ -3' miRNA: 3'- gaUAUUuUCCGCGC-CGGCUuauuugu -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 36029 | 0.68 | 0.999207 |
Target: 5'- aUGUGAAcacGGGCGCGGgCGuuaaAAGCGu -3' miRNA: 3'- gAUAUUU---UCCGCGCCgGCuua-UUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 42782 | 0.68 | 0.999493 |
Target: 5'- -----cGAGGCaGCGuGCCG-AUAAACAu -3' miRNA: 3'- gauauuUUCCG-CGC-CGGCuUAUUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 22754 | 0.68 | 0.999493 |
Target: 5'- uUAUAAAAGGUGCGucacGCCGAu------ -3' miRNA: 3'- gAUAUUUUCCGCGC----CGGCUuauuugu -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 130006 | 0.67 | 0.99981 |
Target: 5'- -------cGGCGCGGCUGAcgaguuUGAAUAu -3' miRNA: 3'- gauauuuuCCGCGCCGGCUu-----AUUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 23751 | 0.67 | 0.999854 |
Target: 5'- gCUGUAuuuAGGGUGCauggGGCCGAAcauuACAc -3' miRNA: 3'- -GAUAUu--UUCCGCG----CCGGCUUauu-UGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 7494 | 0.67 | 0.999889 |
Target: 5'- uUAUuuGGAGGUGuuGCaCGAAUGGACAc -3' miRNA: 3'- gAUAu-UUUCCGCgcCG-GCUUAUUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 107107 | 0.67 | 0.999854 |
Target: 5'- --uUGGAGGGCGaugaccCGGCCGuagaucAUAAACGa -3' miRNA: 3'- gauAUUUUCCGC------GCCGGCu-----UAUUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 108544 | 0.67 | 0.999685 |
Target: 5'- -cGUGAGcgcGGUGCaGCCGAcgGAGCGc -3' miRNA: 3'- gaUAUUUu--CCGCGcCGGCUuaUUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 40353 | 0.67 | 0.99981 |
Target: 5'- cCUGUAGu-GGCaaaGCGGCCGAccaccAUGAAUc -3' miRNA: 3'- -GAUAUUuuCCG---CGCCGGCU-----UAUUUGu -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 70702 | 0.67 | 0.999685 |
Target: 5'- -------uGGCGCuGCCGAGUuAACGg -3' miRNA: 3'- gauauuuuCCGCGcCGGCUUAuUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 125059 | 0.66 | 0.999937 |
Target: 5'- -------cGGCGCGGCgcCGGAUcuGAACGg -3' miRNA: 3'- gauauuuuCCGCGCCG--GCUUA--UUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 28726 | 0.66 | 0.999966 |
Target: 5'- --uUGAAAGGCGCGcauuuGCUGGAgcUAAAUg -3' miRNA: 3'- gauAUUUUCCGCGC-----CGGCUU--AUUUGu -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 112441 | 0.66 | 0.999975 |
Target: 5'- --uUGAAAcGGCGCGGUCGc---AACAc -3' miRNA: 3'- gauAUUUU-CCGCGCCGGCuuauUUGU- -5' |
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22899 | 5' | -45.6 | NC_005137.2 | + | 129404 | 0.66 | 0.999965 |
Target: 5'- uUGUuuAAAGGCGUGcGCCGAcgggucgGUAAAUu -3' miRNA: 3'- gAUAu-UUUCCGCGC-CGGCU-------UAUUUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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