Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22911 | 5' | -52.6 | NC_005137.2 | + | 2496 | 0.7 | 0.833785 |
Target: 5'- -cGC-CGUGuuGAAGGACGUGCGG-GAu -3' miRNA: 3'- aaCGuGCACu-UUUUCUGCGCGCCgCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 6609 | 0.72 | 0.748833 |
Target: 5'- cUGCGCGcUGAAAc-GACGCGCGcGCa- -3' miRNA: 3'- aACGUGC-ACUUUuuCUGCGCGC-CGcu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 8500 | 1.06 | 0.006764 |
Target: 5'- cUUGCACGUGAAAAAGACGCGCGGCGAc -3' miRNA: 3'- -AACGUGCACUUUUUCUGCGCGCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 12864 | 0.68 | 0.915422 |
Target: 5'- -gGCGCaaucUGGAucacGGGCGCGgGGCGAa -3' miRNA: 3'- aaCGUGc---ACUUuu--UCUGCGCgCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 16594 | 0.72 | 0.748833 |
Target: 5'- gUGCGCGcauu---GAUGCGCGGCGGc -3' miRNA: 3'- aACGUGCacuuuuuCUGCGCGCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 25688 | 0.66 | 0.951542 |
Target: 5'- uUUGCAgacaagGUGGAuuGGGACGCGguguCGGCGAg -3' miRNA: 3'- -AACGUg-----CACUUu-UUCUGCGC----GCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 26680 | 0.69 | 0.874442 |
Target: 5'- -cGCACGUGcGAAcguuugauGGCGCugaacgGCGGCGAa -3' miRNA: 3'- aaCGUGCACuUUUu-------CUGCG------CGCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 30720 | 0.67 | 0.927039 |
Target: 5'- cUGCAUGUGGAGAuuguGGGCGUgaauGCcGCGAc -3' miRNA: 3'- aACGUGCACUUUU----UCUGCG----CGcCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 31024 | 0.75 | 0.545563 |
Target: 5'- -gGUGCGUGcgcugcuggcgcacGAAGAGGCGCGCGGCc- -3' miRNA: 3'- aaCGUGCAC--------------UUUUUCUGCGCGCCGcu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 31370 | 0.66 | 0.959568 |
Target: 5'- cUGCaauGCGUGAccaGAAAGGaguugcuugGCGCGGCGc -3' miRNA: 3'- aACG---UGCACU---UUUUCUg--------CGCGCCGCu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 33200 | 0.68 | 0.896059 |
Target: 5'- aUUGCGCGUcGAAGAG-CGCaccGCGGuCGAg -3' miRNA: 3'- -AACGUGCAcUUUUUCuGCG---CGCC-GCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 42057 | 0.66 | 0.959568 |
Target: 5'- --uCACG-GAGAuuGGCGCG-GGCGAa -3' miRNA: 3'- aacGUGCaCUUUuuCUGCGCgCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 44107 | 0.68 | 0.902768 |
Target: 5'- cUGCugGUGcu--GGACGCGUGGg-- -3' miRNA: 3'- aACGugCACuuuuUCUGCGCGCCgcu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 53048 | 0.68 | 0.902768 |
Target: 5'- -gGCAaacacCGUGAAuAAGGUGCGCGGUGu -3' miRNA: 3'- aaCGU-----GCACUUuUUCUGCGCGCCGCu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 70217 | 0.68 | 0.909224 |
Target: 5'- gUGUugGUGAAggcgucgguGAAGGCGU-CGGUGAa -3' miRNA: 3'- aACGugCACUU---------UUUCUGCGcGCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 72292 | 0.72 | 0.718136 |
Target: 5'- uUUGCACGUGugccuAAuuGACGCcaacGCGGCGc -3' miRNA: 3'- -AACGUGCACu----UUuuCUGCG----CGCCGCu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 84292 | 0.74 | 0.60893 |
Target: 5'- -cGCGCGUGGAAAcGGugucgauguccguuGCGCGCGuGCGAg -3' miRNA: 3'- aaCGUGCACUUUU-UC--------------UGCGCGC-CGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 87171 | 0.7 | 0.833785 |
Target: 5'- -cGCACGUGcAAAucgcguucgacGGGCGCG-GGCGGc -3' miRNA: 3'- aaCGUGCACuUUU-----------UCUGCGCgCCGCU- -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 97975 | 0.67 | 0.947155 |
Target: 5'- aUUGUAgGcaAAAcGGGCGCGCGGCGu -3' miRNA: 3'- -AACGUgCacUUUuUCUGCGCGCCGCu -5' |
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22911 | 5' | -52.6 | NC_005137.2 | + | 100034 | 0.69 | 0.850703 |
Target: 5'- -aGCACGcGGuccgacgcGGCGCGCGGCGc -3' miRNA: 3'- aaCGUGCaCUuuuu----CUGCGCGCCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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