Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22911 | 5' | -52.6 | NC_005137.2 | + | 102745 | 0.69 | 0.889098 |
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Target: 5'- -aGCACGU-----AGACGaCGCGGUGGc -3' miRNA: 3'- aaCGUGCAcuuuuUCUGC-GCGCCGCU- -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 108061 | 0.75 | 0.548689 |
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Target: 5'- uUUGCGCGUGGAAcacgcGACGCGgGGCc- -3' miRNA: 3'- -AACGUGCACUUUuu---CUGCGCgCCGcu -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 108688 | 0.81 | 0.282414 |
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Target: 5'- gUGCACGUGAAAaccgugcAAGcCGCGCGGCa- -3' miRNA: 3'- aACGUGCACUUU-------UUCuGCGCGCCGcu -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 125028 | 0.66 | 0.963216 |
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Target: 5'- gUUGCgGCGUGGcgcaggcaacgGAGccGGGCgGCGCGGCGc -3' miRNA: 3'- -AACG-UGCACU-----------UUU--UCUG-CGCGCCGCu -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 125761 | 0.68 | 0.909224 |
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Target: 5'- -cGCGCGUuucGAgcGGCGCGUGGCa- -3' miRNA: 3'- aaCGUGCAcu-UUuuCUGCGCGCCGcu -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 126793 | 0.68 | 0.921361 |
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Target: 5'- aUUGCACGUuuuGAAGcaGCGCGCGGaCa- -3' miRNA: 3'- -AACGUGCAcuuUUUC--UGCGCGCC-Gcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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