Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22911 | 5' | -52.6 | NC_005137.2 | + | 30720 | 0.67 | 0.927039 |
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Target: 5'- cUGCAUGUGGAGAuuguGGGCGUgaauGCcGCGAc -3' miRNA: 3'- aACGUGCACUUUU----UCUGCG----CGcCGCU- -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 97975 | 0.67 | 0.947155 |
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Target: 5'- aUUGUAgGcaAAAcGGGCGCGCGGCGu -3' miRNA: 3'- -AACGUgCacUUUuUCUGCGCGCCGCu -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 25688 | 0.66 | 0.951542 |
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Target: 5'- uUUGCAgacaagGUGGAuuGGGACGCGguguCGGCGAg -3' miRNA: 3'- -AACGUg-----CACUUu-UUCUGCGC----GCCGCU- -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 42057 | 0.66 | 0.959568 |
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Target: 5'- --uCACG-GAGAuuGGCGCG-GGCGAa -3' miRNA: 3'- aacGUGCaCUUUuuCUGCGCgCCGCU- -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 31370 | 0.66 | 0.959568 |
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Target: 5'- cUGCaauGCGUGAccaGAAAGGaguugcuugGCGCGGCGc -3' miRNA: 3'- aACG---UGCACU---UUUUCUg--------CGCGCCGCu -5' |
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| 22911 | 5' | -52.6 | NC_005137.2 | + | 125028 | 0.66 | 0.963216 |
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Target: 5'- gUUGCgGCGUGGcgcaggcaacgGAGccGGGCgGCGCGGCGc -3' miRNA: 3'- -AACG-UGCACU-----------UUU--UCUG-CGCGCCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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