Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22912 | 5' | -55.8 | NC_005137.2 | + | 2374 | 0.69 | 0.712212 |
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Target: 5'- uGGUACGCCguGgGCGUGcUAACCUa- -3' miRNA: 3'- uUCGUGCGGguCgUGCGC-AUUGGAac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 96133 | 0.69 | 0.681123 |
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Target: 5'- cGGUACGCCCAugcGCAuCGCGUuGCCc-- -3' miRNA: 3'- uUCGUGCGGGU---CGU-GCGCAuUGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 50424 | 0.7 | 0.653857 |
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Target: 5'- aAAGCAUGCCUAGCAacuauuugcuaaacaUGCGcGGCCUa- -3' miRNA: 3'- -UUCGUGCGGGUCGU---------------GCGCaUUGGAac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 66880 | 0.7 | 0.628575 |
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Target: 5'- -cGCACGUCauaAGCACGCGgucaaaGACCUg- -3' miRNA: 3'- uuCGUGCGGg--UCGUGCGCa-----UUGGAac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 102760 | 0.7 | 0.626467 |
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Target: 5'- aAGGCACGUgCGGUGCGUGUugggcguucgcuACCUUGg -3' miRNA: 3'- -UUCGUGCGgGUCGUGCGCAu-----------UGGAAC- -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 102954 | 0.71 | 0.597008 |
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Target: 5'- uGGCGCauCCCAGCGCGCGUcgcGCCa-- -3' miRNA: 3'- uUCGUGc-GGGUCGUGCGCAu--UGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 67670 | 0.72 | 0.494616 |
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Target: 5'- uAAGCguGCGuUCCAGCGCGCGUGuguuGCCUUc -3' miRNA: 3'- -UUCG--UGC-GGGUCGUGCGCAU----UGGAAc -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 88534 | 0.77 | 0.283536 |
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Target: 5'- cGGGUucaGCGCCCAGUugGCGUAGCUa-- -3' miRNA: 3'- -UUCG---UGCGGGUCGugCGCAUUGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 8289 | 1.06 | 0.002947 |
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Target: 5'- uAAGCACGCCCAGCACGCGUAACCUUGg -3' miRNA: 3'- -UUCGUGCGGGUCGUGCGCAUUGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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