Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22912 | 5' | -55.8 | NC_005137.2 | + | 63441 | 0.66 | 0.833398 |
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Target: 5'- -cGCAaacaauuuuguuccCGCUCGGCGCGCGUuuguGCCg-- -3' miRNA: 3'- uuCGU--------------GCGGGUCGUGCGCAu---UGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 99367 | 0.66 | 0.835955 |
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Target: 5'- uGGuCGCGUCC-GCGCGCGUgcacuGACCgUUGg -3' miRNA: 3'- uUC-GUGCGGGuCGUGCGCA-----UUGG-AAC- -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 87083 | 0.66 | 0.835955 |
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Target: 5'- aAGGCaugaACGCCguGCACGCG--GCCg-- -3' miRNA: 3'- -UUCG----UGCGGguCGUGCGCauUGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 82867 | 0.66 | 0.835955 |
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Target: 5'- gGAGUuaaACGCCgCAGaaaACGCGUGccGCCUUu -3' miRNA: 3'- -UUCG---UGCGG-GUCg--UGCGCAU--UGGAAc -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 385 | 0.66 | 0.844351 |
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Target: 5'- -uGCugGCUuuguCGGCGCGCGUGuCCg-- -3' miRNA: 3'- uuCGugCGG----GUCGUGCGCAUuGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 66695 | 0.66 | 0.852543 |
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Target: 5'- cGGGCAaauugGCgCAGCGCGCGUcACCc-- -3' miRNA: 3'- -UUCGUg----CGgGUCGUGCGCAuUGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 34004 | 0.66 | 0.852543 |
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Target: 5'- -uGCACGCUUAGCAC-CGU-GCCg-- -3' miRNA: 3'- uuCGUGCGGGUCGUGcGCAuUGGaac -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 65264 | 0.66 | 0.860523 |
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Target: 5'- cAGCGaGCCCGGCGgCGacgGUGACCaUUGg -3' miRNA: 3'- uUCGUgCGGGUCGU-GCg--CAUUGG-AAC- -5' |
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| 22912 | 5' | -55.8 | NC_005137.2 | + | 119580 | 0.66 | 0.868284 |
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Target: 5'- -cGCACGCUgAGCACGUGccACaCUUc -3' miRNA: 3'- uuCGUGCGGgUCGUGCGCauUG-GAAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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