Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22917 | 5' | -53.8 | NC_005137.2 | + | 7695 | 1.1 | 0.002947 |
Target: 5'- gGCACGUAAUGCCUGCGGCGCAUGACUg -3' miRNA: 3'- -CGUGCAUUACGGACGCCGCGUACUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 5400 | 0.75 | 0.518761 |
Target: 5'- cGCGCGUuu--CgaGCGGCGCGUGGCa -3' miRNA: 3'- -CGUGCAuuacGgaCGCCGCGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 67695 | 0.75 | 0.518761 |
Target: 5'- cGCGCGUuuGUGCCgccucGCGGCGU-UGACa -3' miRNA: 3'- -CGUGCAu-UACGGa----CGCCGCGuACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 129992 | 0.73 | 0.612119 |
Target: 5'- gGUACGU--UGUCUcGCGGCGCGgcUGACg -3' miRNA: 3'- -CGUGCAuuACGGA-CGCCGCGU--ACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 1997 | 0.73 | 0.612119 |
Target: 5'- cCGCGUGcacagcucGUGCacgCUGCGGCGCA-GACUa -3' miRNA: 3'- cGUGCAU--------UACG---GACGCCGCGUaCUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100058 | 0.73 | 0.620561 |
Target: 5'- gGCGCGUucagcaugcuuuGUGCC-GCGGCGCGcGGCa -3' miRNA: 3'- -CGUGCAu-----------UACGGaCGCCGCGUaCUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 131047 | 0.71 | 0.727139 |
Target: 5'- uGUACGUAAaa-CUGCGGCGCGUccgcGGCg -3' miRNA: 3'- -CGUGCAUUacgGACGCCGCGUA----CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 32881 | 0.7 | 0.795648 |
Target: 5'- cGCGCGUAcgacgacgcgGUaGCCgGCGGCG-GUGACa -3' miRNA: 3'- -CGUGCAU----------UA-CGGaCGCCGCgUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 12219 | 0.69 | 0.840075 |
Target: 5'- gGCgACGUGAuUGCCgaccgacugGCGuaucaauuGCGCAUGACUa -3' miRNA: 3'- -CG-UGCAUU-ACGGa--------CGC--------CGCGUACUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 20957 | 0.69 | 0.813955 |
Target: 5'- -uGCGUcugcUGCCUGCGGCGCGc---- -3' miRNA: 3'- cgUGCAuu--ACGGACGCCGCGUacuga -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 96493 | 0.68 | 0.886695 |
Target: 5'- uGCGCGUcGUGUCcacgGGCGCuAUGACUa -3' miRNA: 3'- -CGUGCAuUACGGacg-CCGCG-UACUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100407 | 0.68 | 0.886695 |
Target: 5'- cGCGCGcccGUGCgUUGCGGCGCccgGAUc -3' miRNA: 3'- -CGUGCau-UACG-GACGCCGCGua-CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 72294 | 0.68 | 0.863607 |
Target: 5'- uGCACGU-GUGCCUaauugacgccaacGCGGCGCc--ACUg -3' miRNA: 3'- -CGUGCAuUACGGA-------------CGCCGCGuacUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 104616 | 0.68 | 0.856496 |
Target: 5'- aCACGUGcaacaagucGUGgCgCUGCGGCGUGUcGACUg -3' miRNA: 3'- cGUGCAU---------UAC-G-GACGCCGCGUA-CUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 63363 | 0.68 | 0.859678 |
Target: 5'- uGCGCaUAAUGCCUucagacgaauuggagGCGuuGCAUGACa -3' miRNA: 3'- -CGUGcAUUACGGA---------------CGCcgCGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 101159 | 0.68 | 0.863607 |
Target: 5'- cCAUGUAcgGCC-GCGGUuggacagGCGUGGCa -3' miRNA: 3'- cGUGCAUuaCGGaCGCCG-------CGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 31039 | 0.68 | 0.872053 |
Target: 5'- --uUGaAGUGgCUGCGGCGCAUuGGCg -3' miRNA: 3'- cguGCaUUACgGACGCCGCGUA-CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 27942 | 0.68 | 0.872053 |
Target: 5'- cGCACc----GCUUGCGGaaaGCGUGACa -3' miRNA: 3'- -CGUGcauuaCGGACGCCg--CGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 106280 | 0.67 | 0.893658 |
Target: 5'- gGCACGcGAUGCCccaCGGCGCcaAUGuCUg -3' miRNA: 3'- -CGUGCaUUACGGac-GCCGCG--UACuGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 104689 | 0.67 | 0.919044 |
Target: 5'- -aACGUGG-GCC-GCGGCGUGUGGgUg -3' miRNA: 3'- cgUGCAUUaCGGaCGCCGCGUACUgA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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