Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22917 | 5' | -53.8 | NC_005137.2 | + | 131047 | 0.71 | 0.727139 |
Target: 5'- uGUACGUAAaa-CUGCGGCGCGUccgcGGCg -3' miRNA: 3'- -CGUGCAUUacgGACGCCGCGUA----CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 129992 | 0.73 | 0.612119 |
Target: 5'- gGUACGU--UGUCUcGCGGCGCGgcUGACg -3' miRNA: 3'- -CGUGCAuuACGGA-CGCCGCGU--ACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 120104 | 0.66 | 0.930222 |
Target: 5'- -aGCGU--UGCUUGC-GCGUGUGGCUg -3' miRNA: 3'- cgUGCAuuACGGACGcCGCGUACUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 106280 | 0.67 | 0.893658 |
Target: 5'- gGCACGcGAUGCCccaCGGCGCcaAUGuCUg -3' miRNA: 3'- -CGUGCaUUACGGac-GCCGCG--UACuGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 104689 | 0.67 | 0.919044 |
Target: 5'- -aACGUGG-GCC-GCGGCGUGUGGgUg -3' miRNA: 3'- cgUGCAUUaCGGaCGCCGCGUACUgA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 104616 | 0.68 | 0.856496 |
Target: 5'- aCACGUGcaacaagucGUGgCgCUGCGGCGUGUcGACUg -3' miRNA: 3'- cGUGCAU---------UAC-G-GACGCCGCGUA-CUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 101159 | 0.68 | 0.863607 |
Target: 5'- cCAUGUAcgGCC-GCGGUuggacagGCGUGGCa -3' miRNA: 3'- cGUGCAUuaCGGaCGCCG-------CGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100407 | 0.68 | 0.886695 |
Target: 5'- cGCGCGcccGUGCgUUGCGGCGCccgGAUc -3' miRNA: 3'- -CGUGCau-UACG-GACGCCGCGua-CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100141 | 0.66 | 0.935428 |
Target: 5'- gGUugGUGcGUGCgCUGCuGGCGCAcGAa- -3' miRNA: 3'- -CGugCAU-UACG-GACG-CCGCGUaCUga -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100058 | 0.73 | 0.620561 |
Target: 5'- gGCGCGUucagcaugcuuuGUGCC-GCGGCGCGcGGCa -3' miRNA: 3'- -CGUGCAu-----------UACGGaCGCCGCGUaCUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 96493 | 0.68 | 0.886695 |
Target: 5'- uGCGCGUcGUGUCcacgGGCGCuAUGACUa -3' miRNA: 3'- -CGUGCAuUACGGacg-CCGCG-UACUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 95333 | 0.66 | 0.935428 |
Target: 5'- cGUGCGUAGUGCa--CGGCGUcaaugugcgGUGGCa -3' miRNA: 3'- -CGUGCAUUACGgacGCCGCG---------UACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 78065 | 0.66 | 0.935428 |
Target: 5'- uGCAgGUGgguGUGUUUGCGGuUGCAcGACg -3' miRNA: 3'- -CGUgCAU---UACGGACGCC-GCGUaCUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 75977 | 0.67 | 0.913074 |
Target: 5'- cGCGCGuUGGUGCa--CGGCGCAcgGGCc -3' miRNA: 3'- -CGUGC-AUUACGgacGCCGCGUa-CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 72294 | 0.68 | 0.863607 |
Target: 5'- uGCACGU-GUGCCUaauugacgccaacGCGGCGCc--ACUg -3' miRNA: 3'- -CGUGCAuUACGGA-------------CGCCGCGuacUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 70179 | 0.66 | 0.929687 |
Target: 5'- cGUACGcGGUGCCgugggauUGcCGGCGgGUGAUa -3' miRNA: 3'- -CGUGCaUUACGG-------AC-GCCGCgUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 67695 | 0.75 | 0.518761 |
Target: 5'- cGCGCGUuuGUGCCgccucGCGGCGU-UGACa -3' miRNA: 3'- -CGUGCAu-UACGGa----CGCCGCGuACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 63450 | 0.67 | 0.900379 |
Target: 5'- cGCGCGUuGUGCCcGCGaGCGac-GGCg -3' miRNA: 3'- -CGUGCAuUACGGaCGC-CGCguaCUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 63363 | 0.68 | 0.859678 |
Target: 5'- uGCGCaUAAUGCCUucagacgaauuggagGCGuuGCAUGACa -3' miRNA: 3'- -CGUGcAUUACGGA---------------CGCcgCGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 56655 | 0.65 | 0.9491 |
Target: 5'- aCGCGUGcgaccucGUGCUUGCcgacGGCGCugugGACg -3' miRNA: 3'- cGUGCAU-------UACGGACG----CCGCGua--CUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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