Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22919 | 3' | -52.1 | NC_005137.2 | + | 8 | 0.68 | 0.939016 |
Target: 5'- gCGGGgccagugaacaGCGGCGCGUCA--GGcgCAAACu -3' miRNA: 3'- -GCCU-----------UGCCGCGCGGUugUCaaGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 5085 | 0.67 | 0.956703 |
Target: 5'- uGaGACGGCGCGUCGacuuaACAGaggCGAGCu -3' miRNA: 3'- gCcUUGCCGCGCGGU-----UGUCaa-GUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 5266 | 0.68 | 0.933962 |
Target: 5'- ---cGCGGCGCaaGCCG--GGUUCAAGCa -3' miRNA: 3'- gccuUGCCGCG--CGGUugUCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 7352 | 0.66 | 0.976095 |
Target: 5'- uGGAagACGGCacGCGCC---GGUUCGAAa -3' miRNA: 3'- gCCU--UGCCG--CGCGGuugUCAAGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 12702 | 1.1 | 0.004999 |
Target: 5'- uCGGAACGGCGCGCCAACAGUUCAAACu -3' miRNA: 3'- -GCCUUGCCGCGCGGUUGUCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 12808 | 0.68 | 0.933962 |
Target: 5'- cCGGGugGcGUcccacaGCGCCAACGGcgUCAAAg -3' miRNA: 3'- -GCCUugC-CG------CGCGGUUGUCa-AGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 15412 | 0.68 | 0.923082 |
Target: 5'- aCGG-GCGGUGCGUUuu--GUUCGAACg -3' miRNA: 3'- -GCCuUGCCGCGCGGuuguCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 18405 | 0.66 | 0.978573 |
Target: 5'- aCGGAcGCGGCGC-C--GCAG-UCGAACg -3' miRNA: 3'- -GCCU-UGCCGCGcGguUGUCaAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 18484 | 0.66 | 0.970523 |
Target: 5'- ---cGCGGCuGUGCCGACgucaAGUUUAAGCc -3' miRNA: 3'- gccuUGCCG-CGCGGUUG----UCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 21120 | 0.66 | 0.978573 |
Target: 5'- gCGGcaaAAC-GCGCGCCG-CAGgcagCAGACg -3' miRNA: 3'- -GCC---UUGcCGCGCGGUuGUCaa--GUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 21816 | 0.72 | 0.76298 |
Target: 5'- uGGAAaaGCGCGCCGcuuGCAGUugUCAAGa -3' miRNA: 3'- gCCUUgcCGCGCGGU---UGUCA--AGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 22618 | 0.66 | 0.978573 |
Target: 5'- gCGuGAgcGCGGUGCaGCCGACGGagCGcACg -3' miRNA: 3'- -GC-CU--UGCCGCG-CGGUUGUCaaGUuUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 24288 | 0.73 | 0.712359 |
Target: 5'- -cGAACGGCGCGCUGACAGa------ -3' miRNA: 3'- gcCUUGCCGCGCGGUUGUCaaguuug -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 30484 | 0.82 | 0.274332 |
Target: 5'- uGaGGCGGCGCGUCGACAGcUCAAGCg -3' miRNA: 3'- gCcUUGCCGCGCGGUUGUCaAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 31617 | 0.67 | 0.964076 |
Target: 5'- uGGAuuuuAUGGCG-GCCAACGGU-CAGuGCa -3' miRNA: 3'- gCCU----UGCCGCgCGGUUGUCAaGUU-UG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 41612 | 0.67 | 0.964076 |
Target: 5'- gCGGAcaGCGcGUGCGCCAu--GUUUAAAa -3' miRNA: 3'- -GCCU--UGC-CGCGCGGUuguCAAGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 41965 | 0.67 | 0.964076 |
Target: 5'- -cGcgUGGCGCGCCGugACAGcgUCAGAa -3' miRNA: 3'- gcCuuGCCGCGCGGU--UGUCa-AGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 45676 | 0.69 | 0.917255 |
Target: 5'- aCGuGGGCGGCgGCGCCAACg---CGGAUg -3' miRNA: 3'- -GC-CUUGCCG-CGCGGUUGucaaGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 52439 | 0.68 | 0.928651 |
Target: 5'- --uAACGGCGCGCUucCGuUUCAAGCg -3' miRNA: 3'- gccUUGCCGCGCGGuuGUcAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 54891 | 0.66 | 0.967412 |
Target: 5'- gCGGu-UGuGCGCGCCAACGaUUCGuACa -3' miRNA: 3'- -GCCuuGC-CGCGCGGUUGUcAAGUuUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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