Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2293 | 5' | -68 | NC_001408.1 | + | 1034 | 1.1 | 2e-06 |
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Target: 5'- cAGGGCCGGCCCAGGGGCCAGACCCGCg -3' miRNA: 3'- -UCCCGGCCGGGUCCCCGGUCUGGGCG- -5' |
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| 2293 | 5' | -68 | NC_001408.1 | + | 1002 | 0.72 | 0.006516 |
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Target: 5'- cGGGuCUGGCCCcuGGGCCGGcCCUGa -3' miRNA: 3'- uCCC-GGCCGGGucCCCGGUCuGGGCg -5' |
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| 2293 | 5' | -68 | NC_001408.1 | + | 2032 | 0.71 | 0.009608 |
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Target: 5'- cAGGGCCaacGCCCAGGaaaaGGCCucucuucggggucguGGCCCGUc -3' miRNA: 3'- -UCCCGGc--CGGGUCC----CCGGu--------------CUGGGCG- -5' |
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| 2293 | 5' | -68 | NC_001408.1 | + | 2744 | 0.68 | 0.015784 |
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Target: 5'- uGGGGCCGuCCaacagGGGGcGCCAGuucucuccgcgcuCCCGCg -3' miRNA: 3'- -UCCCGGCcGGg----UCCC-CGGUCu------------GGGCG- -5' |
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| 2293 | 5' | -68 | NC_001408.1 | + | 4343 | 0.67 | 0.020574 |
|
Target: 5'- uGGaGGCCGGgguaaacCCUAGGGGUUugGGACCCc- -3' miRNA: 3'- -UC-CCGGCC-------GGGUCCCCGG--UCUGGGcg -5' |
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| 2293 | 5' | -68 | NC_001408.1 | + | 1605 | 0.67 | 0.022711 |
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Target: 5'- -aGG-CGGUUgAGGGGUCAGAucuCCCGCc -3' miRNA: 3'- ucCCgGCCGGgUCCCCGGUCU---GGGCG- -5' |
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| 2293 | 5' | -68 | NC_001408.1 | + | 2242 | 0.66 | 0.024515 |
|
Target: 5'- gAGGaCUGGCCCAccgauuGGCCAGugauggaggccgcgaACCCGCa -3' miRNA: 3'- -UCCcGGCCGGGUcc----CCGGUC---------------UGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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