Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22933 | 3' | -53.7 | NC_005137.2 | + | 130406 | 0.66 | 0.950677 |
Target: 5'- cGGCGGUGgCGgCGGCaCGUUugGCGGCGGa -3' miRNA: 3'- -UUGCCACgGCaGUUG-GCAG--UGUUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 56139 | 0.66 | 0.950677 |
Target: 5'- --gGGUGCCGUCGGCCacguUUGCAAUc- -3' miRNA: 3'- uugCCACGGCAGUUGGc---AGUGUUGcu -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 95205 | 0.67 | 0.920789 |
Target: 5'- cAUGGUGCaCGUCAAUcaagcgaaCGUaCGCAAUGAc -3' miRNA: 3'- uUGCCACG-GCAGUUG--------GCA-GUGUUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 110802 | 0.67 | 0.920789 |
Target: 5'- cACGGgcacaagccaaacGCCGUCGgcgcguuugugcacgGCCGUCACcACGGa -3' miRNA: 3'- uUGCCa------------CGGCAGU---------------UGGCAGUGuUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 104018 | 0.67 | 0.908821 |
Target: 5'- -uCGGUGUCGUCcacguuuCUGUC-CGACGAu -3' miRNA: 3'- uuGCCACGGCAGuu-----GGCAGuGUUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 25510 | 0.68 | 0.874615 |
Target: 5'- cAUGGcGCCGUCAcGCuCGUUGCAACGc -3' miRNA: 3'- uUGCCaCGGCAGU-UG-GCAGUGUUGCu -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 68460 | 0.68 | 0.874615 |
Target: 5'- aGGCaGUGguUCGUCGACCGUUGCGACa- -3' miRNA: 3'- -UUGcCAC--GGCAGUUGGCAGUGUUGcu -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 23593 | 0.69 | 0.851319 |
Target: 5'- -uCGGUGCUuucccaGUCuuugaggccGCCGUCGCAAUGAa -3' miRNA: 3'- uuGCCACGG------CAGu--------UGGCAGUGUUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 17113 | 0.69 | 0.834731 |
Target: 5'- uGAUGGUGCCcUCAgcgagccacauuGCCGUCACcAGCa- -3' miRNA: 3'- -UUGCCACGGcAGU------------UGGCAGUG-UUGcu -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 17236 | 0.7 | 0.817368 |
Target: 5'- cAUGGUGCC-UCGacaaaaaggcACCGUCGCGauGCGAu -3' miRNA: 3'- uUGCCACGGcAGU----------UGGCAGUGU--UGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 31821 | 0.72 | 0.711214 |
Target: 5'- cGGCGGUGUCGUCcGCUaugCACAGCGu -3' miRNA: 3'- -UUGCCACGGCAGuUGGca-GUGUUGCu -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 108269 | 0.72 | 0.659336 |
Target: 5'- uGCGGUGCUG-CGAUCGgcgCGCAACGc -3' miRNA: 3'- uUGCCACGGCaGUUGGCa--GUGUUGCu -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 51841 | 0.76 | 0.474902 |
Target: 5'- cAACGGUGCCG-CAACaacugCGUCAgCGACGAc -3' miRNA: 3'- -UUGCCACGGCaGUUG-----GCAGU-GUUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 13202 | 0.76 | 0.446393 |
Target: 5'- cGCGGUGUCGUguccgacgaaaCcACCGUCACGGCGGa -3' miRNA: 3'- uUGCCACGGCA-----------GuUGGCAGUGUUGCU- -5' |
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22933 | 3' | -53.7 | NC_005137.2 | + | 36479 | 1.07 | 0.005783 |
Target: 5'- cAACGGUGCCGUCAACCGUCACAACGAu -3' miRNA: 3'- -UUGCCACGGCAGUUGGCAGUGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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