Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22946 | 3' | -57.3 | NC_005137.2 | + | 7174 | 0.69 | 0.638047 |
Target: 5'- --cGAgGCCGugacGCGCGuGCGCCAC-GUUg -3' miRNA: 3'- uaaCUgCGGU----UGCGC-CGCGGUGaCAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 12971 | 0.71 | 0.515338 |
Target: 5'- uUUGACGCCGuugGCGCugugGGaCGCCACccgGUCc -3' miRNA: 3'- uAACUGCGGU---UGCG----CC-GCGGUGa--CAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 13527 | 0.71 | 0.52928 |
Target: 5'- cGUUGgauucACGCCGaccgucacuuuaauaGCGCGGCGCaACUGUUg -3' miRNA: 3'- -UAAC-----UGCGGU---------------UGCGCCGCGgUGACAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 17022 | 0.67 | 0.730206 |
Target: 5'- aAUUGGCGCaucucgugcagCAACGCGGUGUCAUcaaacacguUGUUg -3' miRNA: 3'- -UAACUGCG-----------GUUGCGCCGCGGUG---------ACAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 18410 | 0.69 | 0.617287 |
Target: 5'- uGUUaACG--GACGCGGCGCCGCaGUCg -3' miRNA: 3'- -UAAcUGCggUUGCGCCGCGGUGaCAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 24473 | 0.7 | 0.565721 |
Target: 5'- uGUUGuguGCGCgGACGCGGUggacugcaacaaGCCGuCUGUCa -3' miRNA: 3'- -UAAC---UGCGgUUGCGCCG------------CGGU-GACAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 27466 | 0.69 | 0.617287 |
Target: 5'- aAUUGACGUC-GCGaCGuGCGCCGCcaGUCa -3' miRNA: 3'- -UAACUGCGGuUGC-GC-CGCGGUGa-CAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 27880 | 0.8 | 0.14597 |
Target: 5'- gGUUGGCGaucgCGugGCGGCGCCACggGUCa -3' miRNA: 3'- -UAACUGCg---GUugCGCCGCGGUGa-CAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 28208 | 0.66 | 0.806394 |
Target: 5'- -cUGGCGCauccCAGCGCGcgucGCGCCAC-GUUc -3' miRNA: 3'- uaACUGCG----GUUGCGC----CGCGGUGaCAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 31571 | 0.66 | 0.797343 |
Target: 5'- --cGGCGUCGACGUGcaGCuGCCACgGUUg -3' miRNA: 3'- uaaCUGCGGUUGCGC--CG-CGGUGaCAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 35350 | 0.69 | 0.614175 |
Target: 5'- --cGAaGCCGacccgaccaacaacACGCaGGCGCCGCUGUg -3' miRNA: 3'- uaaCUgCGGU--------------UGCG-CCGCGGUGACAg -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 38522 | 0.67 | 0.740136 |
Target: 5'- gGUUG-CGCauauuUggUGCGGUcaacGCCACUGUCa -3' miRNA: 3'- -UAACuGCG-----GuuGCGCCG----CGGUGACAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 39650 | 0.67 | 0.749969 |
Target: 5'- uAUUGcUGCCAGgcuaaGCGGCGCCGCa--- -3' miRNA: 3'- -UAACuGCGGUUg----CGCCGCGGUGacag -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 44979 | 0.69 | 0.648424 |
Target: 5'- uUUGACGCCGACaaGGCgGCCGacgUGUUu -3' miRNA: 3'- uAACUGCGGUUGcgCCG-CGGUg--ACAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 45686 | 0.72 | 0.438937 |
Target: 5'- --cGGCGCCAACGCGGaUGCUcUUGUa -3' miRNA: 3'- uaaCUGCGGUUGCGCC-GCGGuGACAg -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 46045 | 0.69 | 0.627665 |
Target: 5'- --cGGCGCCAACGaGGUuaugccGCCGCUG-Ca -3' miRNA: 3'- uaaCUGCGGUUGCgCCG------CGGUGACaG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 46231 | 0.68 | 0.68971 |
Target: 5'- ---cGCGuCCAAaauaGCGGCGCaCGCUGUg -3' miRNA: 3'- uaacUGC-GGUUg---CGCCGCG-GUGACAg -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 46610 | 0.68 | 0.699932 |
Target: 5'- uUUGACGUCGGCGUGcauGUGCCACa--- -3' miRNA: 3'- uAACUGCGGUUGCGC---CGCGGUGacag -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 46823 | 0.67 | 0.759695 |
Target: 5'- -cUGGCGCguGCuuucgauuaaaGCGGCgcugcccuuagcGCCGCUGUCc -3' miRNA: 3'- uaACUGCGguUG-----------CGCCG------------CGGUGACAG- -5' |
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22946 | 3' | -57.3 | NC_005137.2 | + | 47386 | 0.66 | 0.797343 |
Target: 5'- -gUGGCGgCAAU-CaGCGCCugUGUCa -3' miRNA: 3'- uaACUGCgGUUGcGcCGCGGugACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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