Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22954 | 5' | -50 | NC_005137.2 | + | 69168 | 0.66 | 0.994536 |
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Target: 5'- -cGGAuGCGCGccAGAAugGcGUCGCUg -3' miRNA: 3'- ucCCUuUGCGUu-UCUUugC-CAGCGAg -5' |
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| 22954 | 5' | -50 | NC_005137.2 | + | 123371 | 0.68 | 0.979408 |
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Target: 5'- uGGGu-AC-CGGA-AAACGGUCGCUCa -3' miRNA: 3'- uCCCuuUGcGUUUcUUUGCCAGCGAG- -5' |
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| 22954 | 5' | -50 | NC_005137.2 | + | 52602 | 0.69 | 0.953542 |
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Target: 5'- cGGcGGAGGCGCuu-GAAACGGaagCGCg- -3' miRNA: 3'- -UC-CCUUUGCGuuuCUUUGCCa--GCGag -5' |
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| 22954 | 5' | -50 | NC_005137.2 | + | 16377 | 0.7 | 0.949222 |
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Target: 5'- -cGGAAacgugGCGCAAAGAAGCuaugCGCUCg -3' miRNA: 3'- ucCCUU-----UGCGUUUCUUUGcca-GCGAG- -5' |
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| 22954 | 5' | -50 | NC_005137.2 | + | 112476 | 0.7 | 0.949222 |
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Target: 5'- uGGGAAugGUGGAGA-ACGGgaCGCUg -3' miRNA: 3'- uCCCUUugCGUUUCUuUGCCa-GCGAg -5' |
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| 22954 | 5' | -50 | NC_005137.2 | + | 89195 | 1.11 | 0.00657 |
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Target: 5'- uAGGGAAACGCAAAGAAACGGUCGCUCa -3' miRNA: 3'- -UCCCUUUGCGUUUCUUUGCCAGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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