miRNA: 3'- guaCUCGaaaaCGUUG-GCUGUUGCCG- -5'
miRNA: 3'- -GUACUCGaaa-ACGUUGGCUGUUgcCG- -5'
miRNA: 3'- gUACUCGA-AAACGu------------UGGCUGUUGCCG- -5'
miRNA: 3'- -GUACUCGAAAACGUUGGCUGUUGCCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22959 | 3' | -48.3 | NC_005137.2 | + | 19420 | 0.76 | 0.780528 |
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Target: 5'- --aGAGCacg-GCAACaCGGCAACGGUg -3' miRNA: 3'- guaCUCGaaaaCGUUG-GCUGUUGCCG- -5' |
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| 22959 | 3' | -48.3 | NC_005137.2 | + | 22618 | 0.79 | 0.610104 |
|
Target: 5'- gCGUGAGCgcggUGCAGCCGACGGa-GCg -3' miRNA: 3'- -GUACUCGaaa-ACGUUGGCUGUUgcCG- -5' |
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| 22959 | 3' | -48.3 | NC_005137.2 | + | 100890 | 0.79 | 0.605733 |
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Target: 5'- gGUGGGUUaUUUGCuugggagaucgacGCCGACAACGGCg -3' miRNA: 3'- gUACUCGA-AAACGu------------UGGCUGUUGCCG- -5' |
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| 22959 | 3' | -48.3 | NC_005137.2 | + | 106669 | 1.13 | 0.008011 |
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Target: 5'- aCAUGAGCUUUUGCAACCGACAACGGCc -3' miRNA: 3'- -GUACUCGAAAACGUUGGCUGUUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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