Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22959 | 3' | -48.3 | NC_005137.2 | + | 3582 | 0.69 | 0.986416 |
Target: 5'- aCGUaAGcCUUUUGC--CCGGCGAUGGCc -3' miRNA: 3'- -GUAcUC-GAAAACGuuGGCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 19420 | 0.76 | 0.780528 |
Target: 5'- --aGAGCacg-GCAACaCGGCAACGGUg -3' miRNA: 3'- guaCUCGaaaaCGUUG-GCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 19610 | 0.68 | 0.993186 |
Target: 5'- --cGAGCUUUUGCGugUGGucaAACaGCa -3' miRNA: 3'- guaCUCGAAAACGUugGCUg--UUGcCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 22618 | 0.79 | 0.610104 |
Target: 5'- gCGUGAGCgcggUGCAGCCGACGGa-GCg -3' miRNA: 3'- -GUACUCGaaa-ACGUUGGCUGUUgcCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 30785 | 0.66 | 0.998218 |
Target: 5'- --aGAGUUUUUGUcgUCGuCGACGcGCa -3' miRNA: 3'- guaCUCGAAAACGuuGGCuGUUGC-CG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 32892 | 0.72 | 0.925099 |
Target: 5'- --cGAcGCg---GUAGCCGGCGGCGGUg -3' miRNA: 3'- guaCU-CGaaaaCGUUGGCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 33789 | 0.68 | 0.99414 |
Target: 5'- -uUGuGCUgugccaacgUUGCAACUcggagcuuaauGGCGGCGGCa -3' miRNA: 3'- guACuCGAa--------AACGUUGG-----------CUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 34085 | 0.66 | 0.998692 |
Target: 5'- aCGUGcGCgcgaaugccgGCAAacCCGACAACGuGCg -3' miRNA: 3'- -GUACuCGaaaa------CGUU--GGCUGUUGC-CG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 34989 | 0.7 | 0.975383 |
Target: 5'- ----cGUUUgugGCGACgGGCAACGGCc -3' miRNA: 3'- guacuCGAAaa-CGUUGgCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 35617 | 0.69 | 0.986416 |
Target: 5'- -uUGAGCgaUUGUAAaauaacgcCCGACucaAACGGCa -3' miRNA: 3'- guACUCGaaAACGUU--------GGCUG---UUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 36108 | 0.67 | 0.995724 |
Target: 5'- --cGAcGUUuaaUUUGUAACaGGCAACGGCa -3' miRNA: 3'- guaCU-CGA---AAACGUUGgCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 37831 | 0.68 | 0.992981 |
Target: 5'- -cUGAGCgcuagcaucgUGCuGACCGGCGACGcGUu -3' miRNA: 3'- guACUCGaaa-------ACG-UUGGCUGUUGC-CG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 47406 | 0.68 | 0.991998 |
Target: 5'- gGUGAucaguuuGCgUUUGUacGACCG-CAGCGGCg -3' miRNA: 3'- gUACU-------CGaAAACG--UUGGCuGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 48739 | 0.69 | 0.986416 |
Target: 5'- cCAUGccGCUcUUGUcGCCGAacaaGGCGGCg -3' miRNA: 3'- -GUACu-CGAaAACGuUGGCUg---UUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 49841 | 0.66 | 0.998743 |
Target: 5'- aCAUGAGCauguugUUUUGCAAacuguuuaaacauCCGuGCAggucuugGCGGCg -3' miRNA: 3'- -GUACUCG------AAAACGUU-------------GGC-UGU-------UGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 50436 | 0.66 | 0.998529 |
Target: 5'- gGUGGGCc---GCGaaaaACUGACcGCGGCu -3' miRNA: 3'- gUACUCGaaaaCGU----UGGCUGuUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 59043 | 0.73 | 0.912995 |
Target: 5'- uGUGAGCguugUGCAAacguUUGugGACGGCu -3' miRNA: 3'- gUACUCGaaa-ACGUU----GGCugUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 60652 | 0.75 | 0.834202 |
Target: 5'- -uUGAGCacgUGUuugauuuggccaccGACCGACAGCGGUg -3' miRNA: 3'- guACUCGaaaACG--------------UUGGCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 61348 | 0.69 | 0.984204 |
Target: 5'- uGUGGuGCguguacauugUGCGGCgaGACGACGGCa -3' miRNA: 3'- gUACU-CGaaa-------ACGUUGg-CUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 63008 | 0.66 | 0.998529 |
Target: 5'- cCAUGGuGCg---GC-GCCGGCGuguCGGCa -3' miRNA: 3'- -GUACU-CGaaaaCGuUGGCUGUu--GCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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