Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22959 | 3' | -48.3 | NC_005137.2 | + | 49841 | 0.66 | 0.998743 |
Target: 5'- aCAUGAGCauguugUUUUGCAAacuguuuaaacauCCGuGCAggucuugGCGGCg -3' miRNA: 3'- -GUACUCG------AAAACGUU-------------GGC-UGU-------UGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 79574 | 0.67 | 0.994983 |
Target: 5'- aAUGAGCUUgcGCGccGCCGuGCGAUaGCg -3' miRNA: 3'- gUACUCGAAaaCGU--UGGC-UGUUGcCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 97658 | 0.67 | 0.994983 |
Target: 5'- gAUGGGCacuuugUUUUGCAcCaaauaGGCAAUGGCu -3' miRNA: 3'- gUACUCG------AAAACGUuGg----CUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 78756 | 0.67 | 0.997431 |
Target: 5'- --cGGGCgccgUUUGUAACCagaGCuucuGCGGCg -3' miRNA: 3'- guaCUCGa---AAACGUUGGc--UGu---UGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 30785 | 0.66 | 0.998218 |
Target: 5'- --aGAGUUUUUGUcgUCGuCGACGcGCa -3' miRNA: 3'- guaCUCGAAAACGuuGGCuGUUGC-CG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 75284 | 0.66 | 0.998218 |
Target: 5'- ---cGGCUguaaCGGCgGGCAACGGCg -3' miRNA: 3'- guacUCGAaaacGUUGgCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 80228 | 0.66 | 0.998218 |
Target: 5'- gGUGAacgGCUgcgcggUUGCGuUUGAUGACGGCg -3' miRNA: 3'- gUACU---CGAa-----AACGUuGGCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 63008 | 0.66 | 0.998529 |
Target: 5'- cCAUGGuGCg---GC-GCCGGCGuguCGGCa -3' miRNA: 3'- -GUACU-CGaaaaCGuUGGCUGUu--GCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 96293 | 0.66 | 0.998529 |
Target: 5'- --gGAcGCg--UGCAuucGCCGACAcgcCGGCg -3' miRNA: 3'- guaCU-CGaaaACGU---UGGCUGUu--GCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 33789 | 0.68 | 0.99414 |
Target: 5'- -uUGuGCUgugccaacgUUGCAACUcggagcuuaauGGCGGCGGCa -3' miRNA: 3'- guACuCGAa--------AACGUUGG-----------CUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 87086 | 0.68 | 0.992112 |
Target: 5'- gCAUGAacGCcg-UGCAcgcgGCCGuuaACAACGGCc -3' miRNA: 3'- -GUACU--CGaaaACGU----UGGC---UGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 48739 | 0.69 | 0.986416 |
Target: 5'- cCAUGccGCUcUUGUcGCCGAacaaGGCGGCg -3' miRNA: 3'- -GUACu-CGAaAACGuUGGCUg---UUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 110407 | 0.75 | 0.845551 |
Target: 5'- aCGUGGuGUUUccGCAGCUGGCAACGGg -3' miRNA: 3'- -GUACU-CGAAaaCGUUGGCUGUUGCCg -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 130536 | 0.73 | 0.899807 |
Target: 5'- ----cGCUgg-GCAGCCGccGCGGCGGCa -3' miRNA: 3'- guacuCGAaaaCGUUGGC--UGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 74115 | 0.73 | 0.912995 |
Target: 5'- aCGUGuuGGCUUUcGC-GCCGGC-ACGGCa -3' miRNA: 3'- -GUAC--UCGAAAaCGuUGGCUGuUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 110441 | 0.73 | 0.919184 |
Target: 5'- gGUGAGCg--UGCAcACCG-CGGcCGGCg -3' miRNA: 3'- gUACUCGaaaACGU-UGGCuGUU-GCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 32892 | 0.72 | 0.925099 |
Target: 5'- --cGAcGCg---GUAGCCGGCGGCGGUg -3' miRNA: 3'- guaCU-CGaaaaCGUUGGCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 116882 | 0.71 | 0.950586 |
Target: 5'- uGUGcGCUgccccGCGACuUGACAACGGCc -3' miRNA: 3'- gUACuCGAaaa--CGUUG-GCUGUUGCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 74265 | 0.71 | 0.962689 |
Target: 5'- ----cGCgugUUUGCAACUGACAA-GGCg -3' miRNA: 3'- guacuCGa--AAACGUUGGCUGUUgCCG- -5' |
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22959 | 3' | -48.3 | NC_005137.2 | + | 34989 | 0.7 | 0.975383 |
Target: 5'- ----cGUUUgugGCGACgGGCAACGGCc -3' miRNA: 3'- guacuCGAAaa-CGUUGgCUGUUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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