Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22973 | 3' | -49.2 | NC_005137.2 | + | 165 | 0.7 | 0.951747 |
Target: 5'- uGACGCGCCGCuGUUcacuggccccGCGUACu-- -3' miRNA: 3'- cCUGCGCGGCGuCAAaa--------UGCAUGuuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 2519 | 0.7 | 0.955946 |
Target: 5'- cGGAuUGUGCCGuCGGg--UACGUACAc- -3' miRNA: 3'- -CCU-GCGCGGC-GUCaaaAUGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 4213 | 0.66 | 0.995824 |
Target: 5'- -cGCGCGCUGCuucaaaACGUGCAAu -3' miRNA: 3'- ccUGCGCGGCGucaaaaUGCAUGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 7848 | 0.75 | 0.764629 |
Target: 5'- -cAUGCGCCGCAGgcaUUACGUGCc-- -3' miRNA: 3'- ccUGCGCGGCGUCaa-AAUGCAUGuuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 18404 | 0.7 | 0.947288 |
Target: 5'- cGGACgcgGCGCCGCAGUcgaacgACGcGCAc- -3' miRNA: 3'- -CCUG---CGCGGCGUCAaaa---UGCaUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 21206 | 0.67 | 0.994279 |
Target: 5'- uGGAUGCuUCGCAGaguUUUUACGUggGCGAc -3' miRNA: 3'- -CCUGCGcGGCGUC---AAAAUGCA--UGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 22539 | 0.66 | 0.995824 |
Target: 5'- --cUGgGCCGUAGUUUgguCGUACAc- -3' miRNA: 3'- ccuGCgCGGCGUCAAAau-GCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 24430 | 0.67 | 0.993349 |
Target: 5'- -aGCGCGCCGUucg-UUACaGUGCAAu -3' miRNA: 3'- ccUGCGCGGCGucaaAAUG-CAUGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 26519 | 0.69 | 0.970243 |
Target: 5'- cGGCGCugGCUGC-GUUUUGCGUGCc-- -3' miRNA: 3'- cCUGCG--CGGCGuCAAAAUGCAUGuuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 28471 | 0.74 | 0.813116 |
Target: 5'- -aACGCGuuGCGGUcgUUUugGUGCAAu -3' miRNA: 3'- ccUGCGCggCGUCA--AAAugCAUGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 47236 | 0.67 | 0.990878 |
Target: 5'- aGACuGCGCCGCugcggu-CGUACAAAc -3' miRNA: 3'- cCUG-CGCGGCGucaaaauGCAUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 48906 | 0.67 | 0.993349 |
Target: 5'- cGAuCGCGCCGCcuuGUUcgGCG-ACAAGa -3' miRNA: 3'- cCU-GCGCGGCGu--CAAaaUGCaUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 72288 | 0.79 | 0.583024 |
Target: 5'- uGGACGUGCCGCAGcgUUaggcgaACGUACGc- -3' miRNA: 3'- -CCUGCGCGGCGUCaaAA------UGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 72460 | 0.68 | 0.980642 |
Target: 5'- aGugGCGCCGCguuggcgucaauuAGgcacACGUGCAAAc -3' miRNA: 3'- cCugCGCGGCG-------------UCaaaaUGCAUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 73126 | 0.7 | 0.95989 |
Target: 5'- cGGCGuCGUCGUAGUcgUccACGUACAAAg -3' miRNA: 3'- cCUGC-GCGGCGUCAaaA--UGCAUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 77101 | 0.69 | 0.967031 |
Target: 5'- uGACGCGCaCGUAGUagu-CGUACGc- -3' miRNA: 3'- cCUGCGCG-GCGUCAaaauGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 79908 | 0.67 | 0.989819 |
Target: 5'- uGAgGCGCCGCAaGUggucaaugcgUAUGUGCAc- -3' miRNA: 3'- cCUgCGCGGCGU-CAaa--------AUGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 92842 | 0.66 | 0.99701 |
Target: 5'- cGACGCGCC-CAGUUUUu--UACAu- -3' miRNA: 3'- cCUGCGCGGcGUCAAAAugcAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 93690 | 0.68 | 0.984967 |
Target: 5'- uGGACaCGaCGCAGUUUUcggccgacGCGUGCAu- -3' miRNA: 3'- -CCUGcGCgGCGUCAAAA--------UGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 98143 | 0.68 | 0.988362 |
Target: 5'- cGGGCGCacccacGCCGCGcgcccGUUUUGCcUACAAu -3' miRNA: 3'- -CCUGCG------CGGCGU-----CAAAAUGcAUGUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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