Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22973 | 3' | -49.2 | NC_005137.2 | + | 93690 | 0.68 | 0.984967 |
Target: 5'- uGGACaCGaCGCAGUUUUcggccgacGCGUGCAu- -3' miRNA: 3'- -CCUGcGCgGCGUCAAAA--------UGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 98143 | 0.68 | 0.988362 |
Target: 5'- cGGGCGCacccacGCCGCGcgcccGUUUUGCcUACAAu -3' miRNA: 3'- -CCUGCG------CGGCGU-----CAAAAUGcAUGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 79908 | 0.67 | 0.989819 |
Target: 5'- uGAgGCGCCGCAaGUggucaaugcgUAUGUGCAc- -3' miRNA: 3'- cCUgCGCGGCGU-CAaa--------AUGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 47236 | 0.67 | 0.990878 |
Target: 5'- aGACuGCGCCGCugcggu-CGUACAAAc -3' miRNA: 3'- cCUG-CGCGGCGucaaaauGCAUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 112459 | 0.67 | 0.991129 |
Target: 5'- gGGACGCugaUGCAGUUa-ACGUACAc- -3' miRNA: 3'- -CCUGCGcg-GCGUCAAaaUGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 98185 | 0.67 | 0.992303 |
Target: 5'- uGGACcCGCuCGCAGcgcccgACGUGCAc- -3' miRNA: 3'- -CCUGcGCG-GCGUCaaaa--UGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 24430 | 0.67 | 0.993349 |
Target: 5'- -aGCGCGCCGUucg-UUACaGUGCAAu -3' miRNA: 3'- ccUGCGCGGCGucaaAAUG-CAUGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 48906 | 0.67 | 0.993349 |
Target: 5'- cGAuCGCGCCGCcuuGUUcgGCG-ACAAGa -3' miRNA: 3'- cCU-GCGCGGCGu--CAAaaUGCaUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 21206 | 0.67 | 0.994279 |
Target: 5'- uGGAUGCuUCGCAGaguUUUUACGUggGCGAc -3' miRNA: 3'- -CCUGCGcGGCGUC---AAAAUGCA--UGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 112026 | 0.66 | 0.995824 |
Target: 5'- uGACGCGCUGCG-----ACGUGCu-- -3' miRNA: 3'- cCUGCGCGGCGUcaaaaUGCAUGuuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 4213 | 0.66 | 0.995824 |
Target: 5'- -cGCGCGCUGCuucaaaACGUGCAAu -3' miRNA: 3'- ccUGCGCGGCGucaaaaUGCAUGUUu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 22539 | 0.66 | 0.995824 |
Target: 5'- --cUGgGCCGUAGUUUgguCGUACAc- -3' miRNA: 3'- ccuGCgCGGCGUCAAAau-GCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 115307 | 0.66 | 0.996458 |
Target: 5'- cGGGCgauuGCGCCGCGGUUgaACGcugAgGAGc -3' miRNA: 3'- -CCUG----CGCGGCGUCAAaaUGCa--UgUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 106842 | 0.66 | 0.99701 |
Target: 5'- uGGAguUGCGCaCGCAGU---ACGcGCAGAu -3' miRNA: 3'- -CCU--GCGCG-GCGUCAaaaUGCaUGUUU- -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 92842 | 0.66 | 0.99701 |
Target: 5'- cGACGCGCC-CAGUUUUu--UACAu- -3' miRNA: 3'- cCUGCGCGGcGUCAAAAugcAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 108073 | 0.66 | 0.997489 |
Target: 5'- cGGCGCGuuguuCCGCGGUaagcugggcUACGUGCAu- -3' miRNA: 3'- cCUGCGC-----GGCGUCAaa-------AUGCAUGUuu -5' |
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22973 | 3' | -49.2 | NC_005137.2 | + | 100159 | 0.66 | 0.997489 |
Target: 5'- cGGGCGCGCCGggucguCAGcUUUgauuucccaaUGCGUcGCAAAa -3' miRNA: 3'- -CCUGCGCGGC------GUC-AAA----------AUGCA-UGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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