Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23022 | 3' | -51.6 | NC_005178.1 | + | 16984 | 0.66 | 0.808949 |
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Target: 5'- aGGGCGCUgucguccauauccgGGAaug-CC-UCGGCCAGg -3' miRNA: 3'- cCCUGCGA--------------CCUaaaaGGaAGUCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 1331 | 0.66 | 0.808949 |
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Target: 5'- cGGACGCUgccgcuucGGGUUgUCCUcgucaauccagcguUCgGGCCAGa -3' miRNA: 3'- cCCUGCGA--------CCUAAaAGGA--------------AG-UCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 6479 | 0.67 | 0.770652 |
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Target: 5'- cGGA-GCUGGugag-CCagUUCGGCCAGg -3' miRNA: 3'- cCCUgCGACCuaaaaGG--AAGUCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 17809 | 0.67 | 0.749 |
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Target: 5'- cGGGGCGaaauUGGAUcUUCCgacCGGCCc- -3' miRNA: 3'- -CCCUGCg---ACCUAaAAGGaa-GUCGGuc -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 23320 | 0.67 | 0.749 |
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Target: 5'- gGGGACGCccGAaacgUCCUgaaGGCCGGg -3' miRNA: 3'- -CCCUGCGacCUaaa-AGGAag-UCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 5370 | 0.67 | 0.726818 |
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Target: 5'- -aGGCGCUGGAgg-----UCGGCCAGu -3' miRNA: 3'- ccCUGCGACCUaaaaggaAGUCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 2898 | 0.68 | 0.7133 |
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Target: 5'- -uGAUGCUGGAcauggcgcgCC-UCGGCCAGg -3' miRNA: 3'- ccCUGCGACCUaaaa-----GGaAGUCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 15586 | 0.71 | 0.531478 |
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Target: 5'- cGGGugGUUGGcgaacUCUUUGGCCAGa -3' miRNA: 3'- -CCCugCGACCuaaaaGGAAGUCGGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 33859 | 0.76 | 0.254509 |
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Target: 5'- uGGGACGCUGGAUg-UCCUguUCGGugaagaggaCCAGg -3' miRNA: 3'- -CCCUGCGACCUAaaAGGA--AGUC---------GGUC- -5' |
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| 23022 | 3' | -51.6 | NC_005178.1 | + | 2077 | 1.12 | 0.000847 |
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Target: 5'- gGGGACGCUGGAUUUUCCUUCAGCCAGg -3' miRNA: 3'- -CCCUGCGACCUAAAAGGAAGUCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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