Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23025 | 3' | -61.6 | NC_005178.1 | + | 2735 | 0.66 | 0.311657 |
Target: 5'- gCCCUGGGCGa-GGUGGCCaccaaggCGGuCCGc -3' miRNA: 3'- -GGGACUUGCcaCCGCCGGa------GCC-GGUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 2894 | 0.81 | 0.023447 |
Target: 5'- aCCCUGAugcUGGacaUGGCGcGCCUCGGCCAGg -3' miRNA: 3'- -GGGACUu--GCC---ACCGC-CGGAGCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 4499 | 0.78 | 0.037403 |
Target: 5'- aCCUGGGCGGUGGUGGCUUgGGUgGu -3' miRNA: 3'- gGGACUUGCCACCGCCGGAgCCGgUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 4603 | 0.66 | 0.2916 |
Target: 5'- aCCaCUGGGCGGcguccggcucgaugGGCaGGCCggagcguugccgcUCGGCCAGc -3' miRNA: 3'- -GG-GACUUGCCa-------------CCG-CCGG-------------AGCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 5335 | 1.07 | 0.000207 |
Target: 5'- gCCCUGAACGGUGGCGGCCUCGGCCAAc -3' miRNA: 3'- -GGGACUUGCCACCGCCGGAGCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 5728 | 0.79 | 0.034177 |
Target: 5'- cCCCgGAGCagaucgccgcaucGGUGGCGGCCUCGGgCAc -3' miRNA: 3'- -GGGaCUUG-------------CCACCGCCGGAGCCgGUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 5936 | 0.69 | 0.181619 |
Target: 5'- gCgCUGGACGaugGGCGGCCgagGGCCGGu -3' miRNA: 3'- -GgGACUUGCca-CCGCCGGag-CCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 6579 | 0.69 | 0.175396 |
Target: 5'- cCCUUGGccaaccgcaugcgcACGGUGGCGGCggugaccUGGCCGAa -3' miRNA: 3'- -GGGACU--------------UGCCACCGCCGga-----GCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 6698 | 0.68 | 0.207403 |
Target: 5'- gCCUGGACcg-GGCGGCCUCacugcugaGCCAGg -3' miRNA: 3'- gGGACUUGccaCCGCCGGAGc-------CGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 7078 | 0.74 | 0.074701 |
Target: 5'- gCCCUGGGCGa-GGCGGugaCCUCGGUCGAg -3' miRNA: 3'- -GGGACUUGCcaCCGCC---GGAGCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 7410 | 0.7 | 0.158713 |
Target: 5'- aCCUGAugGGcuuccaGcGCGacGCCUCGGCCGg -3' miRNA: 3'- gGGACUugCCa-----C-CGC--CGGAGCCGGUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 12087 | 0.68 | 0.196729 |
Target: 5'- gCCUGGugGaGUGGUcgcguggcgGGCCU-GGCCGAu -3' miRNA: 3'- gGGACUugC-CACCG---------CCGGAgCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 12416 | 0.67 | 0.242459 |
Target: 5'- gCCUGGGCGGcacguugacgGGCGGUUUCGaGCUg- -3' miRNA: 3'- gGGACUUGCCa---------CCGCCGGAGC-CGGuu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 12801 | 0.66 | 0.303362 |
Target: 5'- uCCCUGGA-GGUcGCGGCCgccauguUCGGCg-- -3' miRNA: 3'- -GGGACUUgCCAcCGCCGG-------AGCCGguu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 15024 | 0.68 | 0.230255 |
Target: 5'- gCUU--GCGGaUGGCGGCCUCGaCCAc -3' miRNA: 3'- gGGAcuUGCC-ACCGCCGGAGCcGGUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 16188 | 0.82 | 0.019096 |
Target: 5'- aCCCUgcugcGAGCGGUGacCGGCCUCGGCCAu -3' miRNA: 3'- -GGGA-----CUUGCCACc-GCCGGAGCCGGUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 16640 | 0.69 | 0.175396 |
Target: 5'- aCCagGGGCGGUaaugacguugccauGGCGGCCaggUCGGCCc- -3' miRNA: 3'- gGGa-CUUGCCA--------------CCGCCGG---AGCCGGuu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 17145 | 0.67 | 0.261104 |
Target: 5'- cCCUUGcGCuccaGGUacucaauGGCGGCCUCcGGCCGc -3' miRNA: 3'- -GGGACuUG----CCA-------CCGCCGGAG-CCGGUu -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 18180 | 0.73 | 0.090859 |
Target: 5'- aCCCUGGcgguucggcugcGCGGUGGCgucuaugGGCCg-GGCCAAa -3' miRNA: 3'- -GGGACU------------UGCCACCG-------CCGGagCCGGUU- -5' |
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23025 | 3' | -61.6 | NC_005178.1 | + | 20532 | 0.71 | 0.136182 |
Target: 5'- -gCUGGGCGGUGGCGGCggcgaucugCUCgacguccgcguccugGGCCAGg -3' miRNA: 3'- ggGACUUGCCACCGCCG---------GAG---------------CCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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