Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23026 | 5' | -59.7 | NC_005178.1 | + | 30339 | 0.66 | 0.330667 |
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Target: 5'- uAGCCgcaGCa-CGCUGCCGCCgccGCUg -3' miRNA: 3'- -UCGGaagCGccGCGAUGGCGGaa-CGA- -5' |
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| 23026 | 5' | -59.7 | NC_005178.1 | + | 28288 | 0.66 | 0.347212 |
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Target: 5'- cGGCCUggauagCgGCGGCGCcgcucaggagcgUGCCGCCa-GCa -3' miRNA: 3'- -UCGGAa-----G-CGCCGCG------------AUGGCGGaaCGa -5' |
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| 23026 | 5' | -59.7 | NC_005178.1 | + | 25678 | 0.66 | 0.355704 |
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Target: 5'- uGGCUUUCGCcGCaGCcucgGCCGCUcgUUGCUc -3' miRNA: 3'- -UCGGAAGCGcCG-CGa---UGGCGG--AACGA- -5' |
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| 23026 | 5' | -59.7 | NC_005178.1 | + | 26165 | 0.66 | 0.355704 |
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Target: 5'- cGCCUUuaucaucaucggCGCGGCGgugGCCGCUgcggGCg -3' miRNA: 3'- uCGGAA------------GCGCCGCga-UGGCGGaa--CGa -5' |
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| 23026 | 5' | -59.7 | NC_005178.1 | + | 11744 | 0.66 | 0.355704 |
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Target: 5'- cAGCgaUUCGaCGGUGCgcagGCCGucgaCCUUGCg -3' miRNA: 3'- -UCGg-AAGC-GCCGCGa---UGGC----GGAACGa -5' |
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| 23026 | 5' | -59.7 | NC_005178.1 | + | 20809 | 0.66 | 0.364341 |
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Target: 5'- cGCCcUCGCGGUuuGCU-CgGCCaUGCa -3' miRNA: 3'- uCGGaAGCGCCG--CGAuGgCGGaACGa -5' |
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| 23026 | 5' | -59.7 | NC_005178.1 | + | 5980 | 0.66 | 0.364341 |
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Target: 5'- gAGUCguucCGgGGCGCUGCC-CCagGCUg -3' miRNA: 3'- -UCGGaa--GCgCCGCGAUGGcGGaaCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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