Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23028 | 3' | -57.6 | NC_005178.1 | + | 10853 | 0.66 | 0.506884 |
Target: 5'- -cGCGgCUGGGcucgGCCAGGAugGCGGCgGCGu -3' miRNA: 3'- caUGCgGGCCU----UGGUCCU--CGCUG-UGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 26975 | 0.66 | 0.486051 |
Target: 5'- -cGCGCCUGGAucgcuACCuGGAGgaCGAgGCa -3' miRNA: 3'- caUGCGGGCCU-----UGGuCCUC--GCUgUGc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 3115 | 0.66 | 0.479881 |
Target: 5'- -cAUGUCCGGGcggcggaccuucggcACCAGG-GCGcCACGc -3' miRNA: 3'- caUGCGGGCCU---------------UGGUCCuCGCuGUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 31223 | 0.66 | 0.465634 |
Target: 5'- cGUuCGCCCcGGACUGGG-GCGACAg- -3' miRNA: 3'- -CAuGCGGGcCUUGGUCCuCGCUGUgc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 28982 | 0.67 | 0.455594 |
Target: 5'- -cGCGCCCcuGGCCuGG-GCGGCACc -3' miRNA: 3'- caUGCGGGccUUGGuCCuCGCUGUGc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 23277 | 0.67 | 0.435871 |
Target: 5'- aUGCGCUCG--ACCAGGAGCGucucCAgGa -3' miRNA: 3'- cAUGCGGGCcuUGGUCCUCGCu---GUgC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 16653 | 0.68 | 0.397956 |
Target: 5'- -gACGCCCaGcuuGACCAGGGGCGGuaaugacguugcCAUGg -3' miRNA: 3'- caUGCGGGcC---UUGGUCCUCGCU------------GUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 14644 | 0.68 | 0.388812 |
Target: 5'- --cCGCgCUGGAguucuACCAGGAGCG-CGCc -3' miRNA: 3'- cauGCG-GGCCU-----UGGUCCUCGCuGUGc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 16602 | 0.68 | 0.377134 |
Target: 5'- ---gGCCCGGuccuugaggucgaaGACCAGGcgGGgGGCGCGg -3' miRNA: 3'- caugCGGGCC--------------UUGGUCC--UCgCUGUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 20809 | 0.68 | 0.370066 |
Target: 5'- gGUACGCCCGgcgcucugcuguuGggUCAGG-GCGGCGa- -3' miRNA: 3'- -CAUGCGGGC-------------CuuGGUCCuCGCUGUgc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 26775 | 0.68 | 0.365695 |
Target: 5'- -aACGCCUGGAGCaacugaccaAGGGGCaggccuuccagccggGACGCGc -3' miRNA: 3'- caUGCGGGCCUUGg--------UCCUCG---------------CUGUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 14385 | 0.69 | 0.320786 |
Target: 5'- -aACGUCCGcAGCCcGGAGCaGCGCGa -3' miRNA: 3'- caUGCGGGCcUUGGuCCUCGcUGUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 3003 | 0.69 | 0.312937 |
Target: 5'- cGUGCgcaGCCUGGAgcgcuuccuggACCAGGccgAGCGugGCGc -3' miRNA: 3'- -CAUG---CGGGCCU-----------UGGUCC---UCGCugUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 21941 | 0.69 | 0.312937 |
Target: 5'- -aGCGCCgGGccGACCAGGAGCacguuggggGAgACGu -3' miRNA: 3'- caUGCGGgCC--UUGGUCCUCG---------CUgUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 23443 | 0.69 | 0.305234 |
Target: 5'- -gACGCCCaGGGcgacgGCCAGGGcgccCGGCGCGa -3' miRNA: 3'- caUGCGGG-CCU-----UGGUCCUc---GCUGUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 22741 | 0.7 | 0.297678 |
Target: 5'- -gACGuCCUGGAacACCAGGAGCcGCAa- -3' miRNA: 3'- caUGC-GGGCCU--UGGUCCUCGcUGUgc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 28296 | 0.7 | 0.290267 |
Target: 5'- -gGCGCugcgggagcugCCGGGGCCGcuGGAGUGGCugGu -3' miRNA: 3'- caUGCG-----------GGCCUUGGU--CCUCGCUGugC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 29042 | 0.7 | 0.283002 |
Target: 5'- gGUGcCGCCCaG-GCCAGGGGCG-CGCa -3' miRNA: 3'- -CAU-GCGGGcCuUGGUCCUCGCuGUGc -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 16917 | 0.7 | 0.283002 |
Target: 5'- -cGCGgCUGaucacauCCAGGAGCGGCGCGa -3' miRNA: 3'- caUGCgGGCcuu----GGUCCUCGCUGUGC- -5' |
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23028 | 3' | -57.6 | NC_005178.1 | + | 3756 | 0.7 | 0.275881 |
Target: 5'- -gACGCgCCGGGccaucGCCAaGGGCGGCACc -3' miRNA: 3'- caUGCG-GGCCU-----UGGUcCUCGCUGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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