miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23028 3' -57.6 NC_005178.1 + 20578 0.7 0.26684
Target:  5'- -gGCGCCCacuucgccgggucgGGGuuACCGGGGGCGGCGa- -3'
miRNA:   3'- caUGCGGG--------------CCU--UGGUCCUCGCUGUgc -5'
23028 3' -57.6 NC_005178.1 + 18100 0.71 0.253399
Target:  5'- -aGCGCCUGGcccAGCCcgccauggugcuguGGGAGCGugGCa -3'
miRNA:   3'- caUGCGGGCC---UUGG--------------UCCUCGCugUGc -5'
23028 3' -57.6 NC_005178.1 + 7339 0.71 0.236144
Target:  5'- --uCGCCUGGAGCCGacaGAGgGGCGCGu -3'
miRNA:   3'- cauGCGGGCCUUGGUc--CUCgCUGUGC- -5'
23028 3' -57.6 NC_005178.1 + 31318 0.71 0.230007
Target:  5'- aUGCGCCUGGcgccgcGCCGGGAGUucGAgGCGa -3'
miRNA:   3'- cAUGCGGGCCu-----UGGUCCUCG--CUgUGC- -5'
23028 3' -57.6 NC_005178.1 + 35604 0.72 0.212402
Target:  5'- ---gGCCCGGAAgCuGGGGCGACGg- -3'
miRNA:   3'- caugCGGGCCUUgGuCCUCGCUGUgc -5'
23028 3' -57.6 NC_005178.1 + 17183 0.74 0.144905
Target:  5'- -cACGCCUGGAugugcuCCAGGAcauccGCGACGCc -3'
miRNA:   3'- caUGCGGGCCUu-----GGUCCU-----CGCUGUGc -5'
23028 3' -57.6 NC_005178.1 + 8287 1.08 0.000479
Target:  5'- gGUACGCCCGGAACCAGGAGCGACACGu -3'
miRNA:   3'- -CAUGCGGGCCUUGGUCCUCGCUGUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.