Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23028 | 3' | -57.6 | NC_005178.1 | + | 28296 | 0.7 | 0.290267 |
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Target: 5'- -gGCGCugcgggagcugCCGGGGCCGcuGGAGUGGCugGu -3' miRNA: 3'- caUGCG-----------GGCCUUGGU--CCUCGCUGugC- -5' |
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| 23028 | 3' | -57.6 | NC_005178.1 | + | 22741 | 0.7 | 0.297678 |
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Target: 5'- -gACGuCCUGGAacACCAGGAGCcGCAa- -3' miRNA: 3'- caUGC-GGGCCU--UGGUCCUCGcUGUgc -5' |
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| 23028 | 3' | -57.6 | NC_005178.1 | + | 23443 | 0.69 | 0.305234 |
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Target: 5'- -gACGCCCaGGGcgacgGCCAGGGcgccCGGCGCGa -3' miRNA: 3'- caUGCGGG-CCU-----UGGUCCUc---GCUGUGC- -5' |
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| 23028 | 3' | -57.6 | NC_005178.1 | + | 3003 | 0.69 | 0.312937 |
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Target: 5'- cGUGCgcaGCCUGGAgcgcuuccuggACCAGGccgAGCGugGCGc -3' miRNA: 3'- -CAUG---CGGGCCU-----------UGGUCC---UCGCugUGC- -5' |
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| 23028 | 3' | -57.6 | NC_005178.1 | + | 14385 | 0.69 | 0.320786 |
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Target: 5'- -aACGUCCGcAGCCcGGAGCaGCGCGa -3' miRNA: 3'- caUGCGGGCcUUGGuCCUCGcUGUGC- -5' |
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| 23028 | 3' | -57.6 | NC_005178.1 | + | 26775 | 0.68 | 0.365695 |
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Target: 5'- -aACGCCUGGAGCaacugaccaAGGGGCaggccuuccagccggGACGCGc -3' miRNA: 3'- caUGCGGGCCUUGg--------UCCUCG---------------CUGUGC- -5' |
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| 23028 | 3' | -57.6 | NC_005178.1 | + | 17183 | 0.74 | 0.144905 |
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Target: 5'- -cACGCCUGGAugugcuCCAGGAcauccGCGACGCc -3' miRNA: 3'- caUGCGGGCCUu-----GGUCCU-----CGCUGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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