Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23033 | 5' | -58.7 | NC_005178.1 | + | 7146 | 0.68 | 0.319551 |
Target: 5'- cCCCGUCGCGau-GCGcuGCUGGaAAGCg -3' miRNA: 3'- uGGGCGGCGUaucCGCu-CGACC-UUCG- -5' |
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23033 | 5' | -58.7 | NC_005178.1 | + | 2730 | 0.68 | 0.30398 |
Target: 5'- --aCGCCGCccUGGGCGAGgUGGccaccaAGGCg -3' miRNA: 3'- uggGCGGCGu-AUCCGCUCgACC------UUCG- -5' |
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23033 | 5' | -58.7 | NC_005178.1 | + | 34164 | 0.82 | 0.030703 |
Target: 5'- gGCCCGCaCGCGaauggacgAGGCGAGCUGGcGGGCc -3' miRNA: 3'- -UGGGCG-GCGUa-------UCCGCUCGACC-UUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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