Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23040 | 3' | -56.4 | NC_005178.1 | + | 31982 | 0.74 | 0.157587 |
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Target: 5'- -aUGCC-GAuccagcgauGGCCGaUGACCGGCAGCGc -3' miRNA: 3'- cgACGGuCU---------UCGGC-AUUGGCCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 5803 | 0.69 | 0.33433 |
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Target: 5'- -gUGCCcGAGGCCGccACCGauGCGGCGa -3' miRNA: 3'- cgACGGuCUUCGGCauUGGC--CGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 3432 | 0.69 | 0.351358 |
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Target: 5'- --gGCCGGAagggccGGCCGUGcAUCGGguGCu -3' miRNA: 3'- cgaCGGUCU------UCGGCAU-UGGCCguCGu -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 10097 | 0.69 | 0.3601 |
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Target: 5'- cGCUgGCCAGGAGCgCgGUGAUuuUGcGCAGCAc -3' miRNA: 3'- -CGA-CGGUCUUCG-G-CAUUG--GC-CGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 16266 | 0.68 | 0.377124 |
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Target: 5'- cGCUGCgGcauggccGAGGCCGgucACCGcucGCAGCAg -3' miRNA: 3'- -CGACGgU-------CUUCGGCau-UGGC---CGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 10336 | 0.67 | 0.425418 |
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Target: 5'- aGCUGCUcGAcuaccaccAG-CGUGGCCGGCAGg- -3' miRNA: 3'- -CGACGGuCU--------UCgGCAUUGGCCGUCgu -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 4811 | 0.67 | 0.434317 |
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Target: 5'- --aGCCGGAcgGGCCGUagaagcaGACCaGGCcgGGCAa -3' miRNA: 3'- cgaCGGUCU--UCGGCA-------UUGG-CCG--UCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 30197 | 0.65 | 0.537427 |
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Target: 5'- cGCuUGUUgaGGGAGCCGaucaUcacuggacggaacuGACCGGCAGCGa -3' miRNA: 3'- -CG-ACGG--UCUUCGGC----A--------------UUGGCCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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