Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23040 | 3' | -56.4 | NC_005178.1 | + | 16646 | 0.66 | 0.518834 |
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Target: 5'- aGCuUGaCCAGggGCgGUAAUgacguugccaUGGCGGCc -3' miRNA: 3'- -CG-AC-GGUCuuCGgCAUUG----------GCCGUCGu -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 15950 | 0.66 | 0.518834 |
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Target: 5'- uGCgGCCGGuGGUCGaaGGCCuGCGGCAa -3' miRNA: 3'- -CGaCGGUCuUCGGCa-UUGGcCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 10350 | 0.66 | 0.528647 |
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Target: 5'- cGC-GCCGGGcugcgcuGGCCG-AGCUggugGGCAGCGa -3' miRNA: 3'- -CGaCGGUCU-------UCGGCaUUGG----CCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 17589 | 0.66 | 0.529742 |
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Target: 5'- uGCUGCCAGAucgGGUCGUc-CCaGCGGa- -3' miRNA: 3'- -CGACGGUCU---UCGGCAuuGGcCGUCgu -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 12643 | 0.66 | 0.529742 |
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Target: 5'- cGCU-CCAGGcGGCCGUcgAugCGuuGCAGCAg -3' miRNA: 3'- -CGAcGGUCU-UCGGCA--UugGC--CGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 35443 | 0.66 | 0.529742 |
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Target: 5'- cGCUGaCCGGGcgccaagguaAGCCGUAcccgcCCGGcCAGUu -3' miRNA: 3'- -CGAC-GGUCU----------UCGGCAUu----GGCC-GUCGu -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 8004 | 0.66 | 0.529742 |
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Target: 5'- gGCUGCCGG-GGCUGacccugacACCGGCAuCGa -3' miRNA: 3'- -CGACGGUCuUCGGCau------UGGCCGUcGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 30197 | 0.65 | 0.537427 |
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Target: 5'- cGCuUGUUgaGGGAGCCGaucaUcacuggacggaacuGACCGGCAGCGa -3' miRNA: 3'- -CG-ACGG--UCUUCGGC----A--------------UUGGCCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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