Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23040 | 3' | -56.4 | NC_005178.1 | + | 10831 | 0.72 | 0.208236 |
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Target: 5'- uCUGCUGGAAGCCugcccgGUcAACUGGCAGCc -3' miRNA: 3'- cGACGGUCUUCGG------CA-UUGGCCGUCGu -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 31982 | 0.74 | 0.157587 |
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Target: 5'- -aUGCC-GAuccagcgauGGCCGaUGACCGGCAGCGc -3' miRNA: 3'- cgACGGuCU---------UCGGC-AUUGGCCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 25842 | 0.74 | 0.153185 |
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Target: 5'- --cGCCAu-GGCCGUGGCCGGCauuucGGCAg -3' miRNA: 3'- cgaCGGUcuUCGGCAUUGGCCG-----UCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 37280 | 0.75 | 0.136673 |
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Target: 5'- gGCUGUCAGGuccggcGCUGU-GCCGGUAGCGa -3' miRNA: 3'- -CGACGGUCUu-----CGGCAuUGGCCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 6882 | 0.75 | 0.132808 |
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Target: 5'- aCUGCCAGGcaaugAGgCGgcgGAUCGGCAGCAg -3' miRNA: 3'- cGACGGUCU-----UCgGCa--UUGGCCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 28307 | 0.76 | 0.114955 |
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Target: 5'- aGCUGCCGG-GGCCGcuggagUGGCUGGUGGCGg -3' miRNA: 3'- -CGACGGUCuUCGGC------AUUGGCCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 20997 | 0.77 | 0.096494 |
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Target: 5'- cGCUaCCGGGuucGCCGUGGgCGGCAGCAc -3' miRNA: 3'- -CGAcGGUCUu--CGGCAUUgGCCGUCGU- -5' |
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| 23040 | 3' | -56.4 | NC_005178.1 | + | 22226 | 1.11 | 0.000297 |
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Target: 5'- aGCUGCCAGAAGCCGUAACCGGCAGCAg -3' miRNA: 3'- -CGACGGUCUUCGGCAUUGGCCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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