Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23041 | 3' | -54 | NC_005178.1 | + | 28538 | 0.69 | 0.483919 |
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Target: 5'- aGCGGAguaAGCccugGCuGCAUGGcGGAUGCCg -3' miRNA: 3'- -CGCCUa--UCGa---CG-CGUGCU-UCUACGGg -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 22381 | 1.14 | 0.000375 |
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Target: 5'- gGCGGAUAGCUGCGCACGAAGAUGCCCu -3' miRNA: 3'- -CGCCUAUCGACGCGUGCUUCUACGGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 6622 | 0.81 | 0.08184 |
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Target: 5'- -aGGAUcGCUGCGCACGGAGGcaaggGCCUg -3' miRNA: 3'- cgCCUAuCGACGCGUGCUUCUa----CGGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 1487 | 0.79 | 0.116524 |
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Target: 5'- cGCGGGccGGCUGCGgAacauCGAAGAUGUCCa -3' miRNA: 3'- -CGCCUa-UCGACGCgU----GCUUCUACGGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 29452 | 0.75 | 0.20465 |
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Target: 5'- cGCGGAgauagcAGCUGCGCuCGuaggggcugcuGAUGCCCc -3' miRNA: 3'- -CGCCUa-----UCGACGCGuGCuu---------CUACGGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 24450 | 0.73 | 0.307127 |
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Target: 5'- aCGGucGGCUGCgGCGCGguGAacaUGCCCa -3' miRNA: 3'- cGCCuaUCGACG-CGUGCuuCU---ACGGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 4422 | 0.73 | 0.307127 |
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Target: 5'- cGCGGc--GCUGCGCGacCGGAGuUGCUCg -3' miRNA: 3'- -CGCCuauCGACGCGU--GCUUCuACGGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 35312 | 0.72 | 0.323209 |
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Target: 5'- uCGGcgcAGCcGCGCGCGAGGGUGaCCg -3' miRNA: 3'- cGCCua-UCGaCGCGUGCUUCUACgGG- -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 16576 | 0.7 | 0.412794 |
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Target: 5'- gGCGGggGGCgcgGCGUGCGucGAgguUGCCg -3' miRNA: 3'- -CGCCuaUCGa--CGCGUGCuuCU---ACGGg -5' |
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| 23041 | 3' | -54 | NC_005178.1 | + | 24796 | 0.66 | 0.696565 |
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Target: 5'- gGUGGGUgAGCUGCuCuuCGAAGGUcaGCUCg -3' miRNA: 3'- -CGCCUA-UCGACGcGu-GCUUCUA--CGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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