Results 21 - 40 of 56 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23044 | 3' | -62.4 | NC_005178.1 | + | 18663 | 0.67 | 0.22272 |
Target: 5'- aGGUGAcuaucCCGGCGCGCCcguaCCUcccgguccucagaaaCGGCCAg -3' miRNA: 3'- -CUACU-----GGCCGCGCGGc---GGAa--------------GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 34662 | 0.67 | 0.222144 |
Target: 5'- --cGACCGcCGCGCCcguaGCCUgaacccUGGCCAg -3' miRNA: 3'- cuaCUGGCcGCGCGG----CGGAa-----GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 35051 | 0.67 | 0.222144 |
Target: 5'- --aGGCUGGUcgGCGCCucgGCCagcgCGGCCAa -3' miRNA: 3'- cuaCUGGCCG--CGCGG---CGGaa--GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 35445 | 0.67 | 0.221568 |
Target: 5'- -cUGACCGG-GCGCCaagguaaGCCguacccgccCGGCCAg -3' miRNA: 3'- cuACUGGCCgCGCGG-------CGGaa-------GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 9723 | 0.67 | 0.216447 |
Target: 5'- --cGGCCGGCucgGCGaaGCCacCGGCCGc -3' miRNA: 3'- cuaCUGGCCG---CGCggCGGaaGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 16899 | 0.67 | 0.216447 |
Target: 5'- --gGAgCGGCGCGaCCagGCUaUCGGCCu -3' miRNA: 3'- cuaCUgGCCGCGC-GG--CGGaAGCCGGu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 34712 | 0.67 | 0.210876 |
Target: 5'- --cGAcCCGGCGCGgcaucccugaaaCCGUCguucgggUCGGCCGg -3' miRNA: 3'- cuaCU-GGCCGCGC------------GGCGGa------AGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 18734 | 0.67 | 0.210876 |
Target: 5'- --gGACCgggagguacgGGCGCGCCGggauagucaCCUUaCGGCCu -3' miRNA: 3'- cuaCUGG----------CCGCGCGGC---------GGAA-GCCGGu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 12791 | 0.67 | 0.209229 |
Target: 5'- --cGACCGGCagucccuggaggucGCgGCCGCCaugUUCGGCg- -3' miRNA: 3'- cuaCUGGCCG--------------CG-CGGCGG---AAGCCGgu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 21071 | 0.68 | 0.200105 |
Target: 5'- cGGUGG-CGGUGCuGCCGCCcaCGGCgAa -3' miRNA: 3'- -CUACUgGCCGCG-CGGCGGaaGCCGgU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 10249 | 0.68 | 0.200105 |
Target: 5'- cAUGGCCGGaCGCauCgGCCUgcCGGCCAc -3' miRNA: 3'- cUACUGGCC-GCGc-GgCGGAa-GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 17834 | 0.68 | 0.181921 |
Target: 5'- --aGGCCGaGCGCacccugggagcgccuGCCGCCcUCGGCgAg -3' miRNA: 3'- cuaCUGGC-CGCG---------------CGGCGGaAGCCGgU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 35092 | 0.68 | 0.180473 |
Target: 5'- --cGACCGGacuacucgccgccgaGCuGCCGCUggCGGCCGg -3' miRNA: 3'- cuaCUGGCCg--------------CG-CGGCGGaaGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 23535 | 0.68 | 0.179993 |
Target: 5'- --cGGCCGagcaGCGgGCCGgCUUCGcGCCGg -3' miRNA: 3'- cuaCUGGC----CGCgCGGCgGAAGC-CGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 11100 | 0.68 | 0.179993 |
Target: 5'- --cGGCCGGCaGCGCCGCgCUgcugaaaauguUUGGCa- -3' miRNA: 3'- cuaCUGGCCG-CGCGGCG-GA-----------AGCCGgu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 26094 | 0.68 | 0.179993 |
Target: 5'- --gGGgCGGaCGCuGCCGCagcgUCGGCCAa -3' miRNA: 3'- cuaCUgGCC-GCG-CGGCGga--AGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 23466 | 0.69 | 0.166105 |
Target: 5'- --cGcCCGGCGCGaaGCCggcccgcugcUCGGCCGg -3' miRNA: 3'- cuaCuGGCCGCGCggCGGa---------AGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 7413 | 0.69 | 0.166105 |
Target: 5'- uGAUGGgcuuCCaGCGCGaCGCC-UCGGCCGg -3' miRNA: 3'- -CUACU----GGcCGCGCgGCGGaAGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 31890 | 0.69 | 0.164771 |
Target: 5'- cGUGACCgacgucgccgggauGGCGCuGCCgGUCaUCGGCCAu -3' miRNA: 3'- cUACUGG--------------CCGCG-CGG-CGGaAGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 33332 | 0.7 | 0.145088 |
Target: 5'- uGUGACgagGGCGacgaacagguCGCCGUCUUCGGUCAg -3' miRNA: 3'- cUACUGg--CCGC----------GCGGCGGAAGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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