Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23048 | 3' | -53.2 | NC_005178.1 | + | 37456 | 0.66 | 0.733811 |
Target: 5'- gGAAGCgAUGGa-GGCCGaguUCAGCc -3' miRNA: 3'- gCUUCGgUACCaaCUGGCgguAGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 27662 | 0.66 | 0.689028 |
Target: 5'- cCGcuGCCGUGGgcaUGGgCGCgCAaCAGCg -3' miRNA: 3'- -GCuuCGGUACCa--ACUgGCG-GUaGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 22262 | 0.66 | 0.689028 |
Target: 5'- cCGAccGCCA-GGcUGGcCCGCCAggCGGCg -3' miRNA: 3'- -GCUu-CGGUaCCaACU-GGCGGUa-GUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 12869 | 0.66 | 0.699217 |
Target: 5'- gGAAGCCGaGGggGACgacugggacaaggCGCaAUCGGCg -3' miRNA: 3'- gCUUCGGUaCCaaCUG-------------GCGgUAGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 25808 | 0.66 | 0.700345 |
Target: 5'- aGGAGCaacGG--GACCGCCugcUCGGCa -3' miRNA: 3'- gCUUCGguaCCaaCUGGCGGu--AGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 35398 | 0.66 | 0.72275 |
Target: 5'- gCGAGGgCCAacUGGccccggcgcUGGCCGCCAccgaCAGCc -3' miRNA: 3'- -GCUUC-GGU--ACCa--------ACUGGCGGUa---GUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 11821 | 0.66 | 0.72275 |
Target: 5'- --uGGCCGcccUGG-UGACCGCgAucauUCGGCa -3' miRNA: 3'- gcuUCGGU---ACCaACUGGCGgU----AGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 14461 | 0.66 | 0.72275 |
Target: 5'- aCGAcaCCAUGGacgUGAUCaUCAUCGGCa -3' miRNA: 3'- -GCUucGGUACCa--ACUGGcGGUAGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 13376 | 0.66 | 0.733811 |
Target: 5'- gGAGG--AUGGUcuUGACCGCUuucugauagcaGUCGGCa -3' miRNA: 3'- gCUUCggUACCA--ACUGGCGG-----------UAGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 24866 | 0.67 | 0.654769 |
Target: 5'- gGAAGCuCGUucugccGUUGAUUGCCGUgAGCu -3' miRNA: 3'- gCUUCG-GUAc-----CAACUGGCGGUAgUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 26354 | 0.68 | 0.605381 |
Target: 5'- uGAGGCCGUGGcgGuacugucggaaaucACCGCgAcCGGCa -3' miRNA: 3'- gCUUCGGUACCaaC--------------UGGCGgUaGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 21009 | 0.68 | 0.585936 |
Target: 5'- ---cGCCGUGGgcGgcagcACCGCCAcCGGCc -3' miRNA: 3'- gcuuCGGUACCaaC-----UGGCGGUaGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 25839 | 0.75 | 0.242329 |
Target: 5'- uGAcGCCAUGGccgUGGCCGgCAuuUCGGCa -3' miRNA: 3'- gCUuCGGUACCa--ACUGGCgGU--AGUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 25131 | 0.72 | 0.36713 |
Target: 5'- gCGAAGCCGaGGcagaaaagGGCCGCCAgcaAGCc -3' miRNA: 3'- -GCUUCGGUaCCaa------CUGGCGGUag-UCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 14944 | 0.72 | 0.379945 |
Target: 5'- gGGAGacaggcguccuggaCGUGGUcgGGCCGCCAUCcGCa -3' miRNA: 3'- gCUUCg-------------GUACCAa-CUGGCGGUAGuCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 11769 | 0.71 | 0.4241 |
Target: 5'- -cGAGCCAUGGggGuuCCGCCG-CAGg -3' miRNA: 3'- gcUUCGGUACCaaCu-GGCGGUaGUCg -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 25940 | 0.71 | 0.434098 |
Target: 5'- gGAuGCCAcgUGGaUGGuuGCCAUcCAGCa -3' miRNA: 3'- gCUuCGGU--ACCaACUggCGGUA-GUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 20362 | 0.7 | 0.496779 |
Target: 5'- aGAGGCCAUugcGGcccUGACCGCCcugaAGCc -3' miRNA: 3'- gCUUCGGUA---CCa--ACUGGCGGuag-UCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 17616 | 0.69 | 0.507629 |
Target: 5'- gCGucGCCGUGGUcUGACgGUCGagucCAGCg -3' miRNA: 3'- -GCuuCGGUACCA-ACUGgCGGUa---GUCG- -5' |
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23048 | 3' | -53.2 | NC_005178.1 | + | 4929 | 0.68 | 0.563219 |
Target: 5'- aGAucGCCGaGGaacUGGCCGCCAgCGGCc -3' miRNA: 3'- gCUu-CGGUaCCa--ACUGGCGGUaGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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