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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23048 | 5' | -57.6 | NC_005178.1 | + | 25200 | 1.07 | 0.000418 |
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Target: 5'- cUUGCUGGCGGCCCUUUUCUGCCUCGGc -3' miRNA: 3'- -AACGACCGCCGGGAAAAGACGGAGCC- -5' |
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| 23048 | 5' | -57.6 | NC_005178.1 | + | 4991 | 0.69 | 0.27364 |
|
Target: 5'- -cGCUGGCGGCCag---UU-CCUCGGc -3' miRNA: 3'- aaCGACCGCCGGgaaaaGAcGGAGCC- -5' |
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| 23048 | 5' | -57.6 | NC_005178.1 | + | 9581 | 0.69 | 0.280852 |
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Target: 5'- -cGCgccaGGCGGCgCUggcggUCUGUCUUGGu -3' miRNA: 3'- aaCGa---CCGCCGgGAaa---AGACGGAGCC- -5' |
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| 23048 | 5' | -57.6 | NC_005178.1 | + | 12669 | 0.68 | 0.319175 |
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Target: 5'- -cGCUGGCGGguggCCUgg-UUGCCUgCGGc -3' miRNA: 3'- aaCGACCGCCg---GGAaaaGACGGA-GCC- -5' |
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| 23048 | 5' | -57.6 | NC_005178.1 | + | 16622 | 0.67 | 0.370167 |
|
Target: 5'- gUUGCcaUGGCGGCCagg-UCgGCC-CGGu -3' miRNA: 3'- -AACG--ACCGCCGGgaaaAGaCGGaGCC- -5' |
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| 23048 | 5' | -57.6 | NC_005178.1 | + | 17109 | 0.67 | 0.37919 |
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Target: 5'- -cGCaUGGCgaagauGGCCCgcagaUCgGCCUCGGu -3' miRNA: 3'- aaCG-ACCG------CCGGGaaa--AGaCGGAGCC- -5' |
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| 23048 | 5' | -57.6 | NC_005178.1 | + | 20431 | 0.66 | 0.435326 |
|
Target: 5'- -gGCUucagGGCGGUCagggccgcaauggccUCUGCCUCGGu -3' miRNA: 3'- aaCGA----CCGCCGGgaaa-----------AGACGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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