Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23049 | 5' | -58.2 | NC_005178.1 | + | 28629 | 0.71 | 0.188086 |
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Target: 5'- gGGAAUGCCGGCgACGcCCccgGUGGCG-Cg -3' miRNA: 3'- -CUUUACGGCCGgUGCcGG---UACCGCaG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 12840 | 0.72 | 0.178007 |
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Target: 5'- gGGAcUGCCGGUCgaggacguaggcGCGGCgCAgGGCGUCg -3' miRNA: 3'- -CUUuACGGCCGG------------UGCCG-GUaCCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 35866 | 0.72 | 0.16379 |
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Target: 5'- ----aGCCGGaaaCCGaGGCCGUGGCGUUc -3' miRNA: 3'- cuuuaCGGCC---GGUgCCGGUACCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 15793 | 0.73 | 0.154886 |
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Target: 5'- ----cGUCGGCCuucucuGCGGCCGaGGCGUUg -3' miRNA: 3'- cuuuaCGGCCGG------UGCCGGUaCCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 10064 | 0.73 | 0.142353 |
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Target: 5'- cGGuuUGCCGGUUuuGCGGCacuuuUGGCGUCg -3' miRNA: 3'- -CUuuACGGCCGG--UGCCGgu---ACCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 28261 | 0.75 | 0.101002 |
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Target: 5'- ----cGCUGGCCGCcGCCaAUGGUGUCa -3' miRNA: 3'- cuuuaCGGCCGGUGcCGG-UACCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 11810 | 0.76 | 0.082402 |
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Target: 5'- ----gGCUcgGGCCACGGCCGUGGCa-- -3' miRNA: 3'- cuuuaCGG--CCGGUGCCGGUACCGcag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 25902 | 1.1 | 0.000224 |
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Target: 5'- cGAAAUGCCGGCCACGGCCAUGGCGUCa -3' miRNA: 3'- -CUUUACGGCCGGUGCCGGUACCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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