Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23055 | 3' | -53.5 | NC_005178.1 | + | 32628 | 0.66 | 0.715113 |
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Target: 5'- gACCCGGucgccGAGCGGUGccgUCGACCUGGu -3' miRNA: 3'- gUGGGCC-----UUCGUUAUu--GGCUGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 33104 | 0.71 | 0.449916 |
|
Target: 5'- cCugCCGGAAuGCccucgguGCCG-CCCAGGa -3' miRNA: 3'- -GugGGCCUU-CGuuau---UGGCuGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 33557 | 0.71 | 0.449916 |
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Target: 5'- cCugCCGGuGGCAGUAGCCcugucgaguGGUCCAGGu -3' miRNA: 3'- -GugGGCCuUCGUUAUUGG---------CUGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 33794 | 0.67 | 0.674809 |
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Target: 5'- uCGCCCGGAuuGCAcaAACUucaucgacuuucuuGCCCAGGg -3' miRNA: 3'- -GUGGGCCUu-CGUuaUUGGc-------------UGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 33907 | 0.72 | 0.390376 |
|
Target: 5'- gCGCCUGGcGGCGAUGcucggcggccgguGCCGGCuuuCCGGGg -3' miRNA: 3'- -GUGGGCCuUCGUUAU-------------UGGCUG---GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 33987 | 0.67 | 0.670286 |
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Target: 5'- -cCCCGGAA-----AGCCGGCaCCAGGc -3' miRNA: 3'- guGGGCCUUcguuaUUGGCUG-GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 34638 | 0.69 | 0.513038 |
|
Target: 5'- uCGCCCGGccAGCuccccGGUGGCaCG-CCCAGGa -3' miRNA: 3'- -GUGGGCCu-UCG-----UUAUUG-GCuGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 35604 | 0.72 | 0.37291 |
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Target: 5'- gGCCCGGAAGCug-GGgCGACggugacgcuCCAGGu -3' miRNA: 3'- gUGGGCCUUCGuuaUUgGCUG---------GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 36098 | 0.72 | 0.355107 |
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Target: 5'- cUAUCUGGGAGUAcauGCCG-CCCAGGu -3' miRNA: 3'- -GUGGGCCUUCGUuauUGGCuGGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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