Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23055 | 5' | -56.4 | NC_005178.1 | + | 32352 | 0.67 | 0.521702 |
Target: 5'- -cUCGAccacGUCGGUGGCGGcgCUacagaGCGCc -3' miRNA: 3'- acGGCUu---UAGCCACCGCCaaGG-----CGCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 5340 | 0.67 | 0.521702 |
Target: 5'- cGCCGGcccuGAaCGGUGGCGGccUCgGC-Ca -3' miRNA: 3'- aCGGCU----UUaGCCACCGCCa-AGgCGcG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 11579 | 0.67 | 0.521702 |
Target: 5'- uUGCCGggGUUGuuGUuGCGGaugCCGCGa -3' miRNA: 3'- -ACGGCuuUAGC--CAcCGCCaa-GGCGCg -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 27034 | 0.66 | 0.554168 |
Target: 5'- cUGCUGGAGUCGGUGcGCaccaacaaGGaaaaGCGCg -3' miRNA: 3'- -ACGGCUUUAGCCAC-CG--------CCaaggCGCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 20952 | 0.66 | 0.554168 |
Target: 5'- gUGuuGcgGUCGGUGGUcaGGgcggUCauaGCGCc -3' miRNA: 3'- -ACggCuuUAGCCACCG--CCa---AGg--CGCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 3456 | 0.66 | 0.565127 |
Target: 5'- gGCCG-AGUCGGcccUGGUGGUggUCGaUGCc -3' miRNA: 3'- aCGGCuUUAGCC---ACCGCCAa-GGC-GCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 28695 | 0.66 | 0.565127 |
Target: 5'- cGCUGAAGUUcuUGGaCGGUUCCuguaucugagcgGCGCc -3' miRNA: 3'- aCGGCUUUAGccACC-GCCAAGG------------CGCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 10636 | 0.66 | 0.576142 |
Target: 5'- aGCCGggGcgcaacgcccCGGccGGCGucUCCGCGCc -3' miRNA: 3'- aCGGCuuUa---------GCCa-CCGCcaAGGCGCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 29908 | 0.66 | 0.576142 |
Target: 5'- aUGCCGAGGcgCGGUcGGUgacccagccaagGGUUUCgGCGUg -3' miRNA: 3'- -ACGGCUUUa-GCCA-CCG------------CCAAGG-CGCG- -5' |
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23055 | 5' | -56.4 | NC_005178.1 | + | 21442 | 0.66 | 0.576142 |
Target: 5'- cUGCCa-----GGUGGUGGgggCCGcCGCg -3' miRNA: 3'- -ACGGcuuuagCCACCGCCaa-GGC-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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