Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23055 | 5' | -56.4 | NC_005178.1 | + | 35476 | 0.69 | 0.373645 |
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Target: 5'- gGCUG---UCGGUGGCGG--CCaGCGCc -3' miRNA: 3'- aCGGCuuuAGCCACCGCCaaGG-CGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 25693 | 0.7 | 0.35615 |
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Target: 5'- gGUCGAAcUCGGcGGUGGcUUUCGcCGCa -3' miRNA: 3'- aCGGCUUuAGCCaCCGCC-AAGGC-GCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 35325 | 0.7 | 0.339239 |
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Target: 5'- gGCCGGua-CGGUaucGGCGcagCCGCGCg -3' miRNA: 3'- aCGGCUuuaGCCA---CCGCcaaGGCGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 27325 | 0.7 | 0.312628 |
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Target: 5'- -cCUGAAAUCGGUuugcaacugguccaGGCGGUUCUuCGCc -3' miRNA: 3'- acGGCUUUAGCCA--------------CCGCCAAGGcGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 34593 | 0.72 | 0.250161 |
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Target: 5'- gGCCGGgcgAGUCGGUGaGCGGgaaaUGCGUc -3' miRNA: 3'- aCGGCU---UUAGCCAC-CGCCaag-GCGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 22393 | 0.72 | 0.237341 |
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Target: 5'- cGCCGGAa--GGUcGGCGGauagCUGCGCa -3' miRNA: 3'- aCGGCUUuagCCA-CCGCCaa--GGCGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 22472 | 0.73 | 0.219147 |
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Target: 5'- uUGCCGGAcgacGUCGGcGGCGaGggugagccgUCCGCGUc -3' miRNA: 3'- -ACGGCUU----UAGCCaCCGC-Ca--------AGGCGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 11031 | 0.73 | 0.217395 |
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Target: 5'- aUGCgGAAAUCGGUGucggugacGCGGggggcaggaggcgggCCGCGCc -3' miRNA: 3'- -ACGgCUUUAGCCAC--------CGCCaa-------------GGCGCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 5741 | 0.76 | 0.133472 |
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Target: 5'- cGCCGc-AUCGGUGGCGGccUCGgGCa -3' miRNA: 3'- aCGGCuuUAGCCACCGCCaaGGCgCG- -5' |
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| 23055 | 5' | -56.4 | NC_005178.1 | + | 30685 | 1.11 | 0.000352 |
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Target: 5'- cUGCCGAAAUCGGUGGCGGUUCCGCGCu -3' miRNA: 3'- -ACGGCUUUAGCCACCGCCAAGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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