Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23057 | 3' | -60.4 | NC_005178.1 | + | 35712 | 0.66 | 0.333956 |
Target: 5'- gGCCGGCGuaGGUgCGCuucagcgucgUGCCgcuguaGACCUg -3' miRNA: 3'- -CGGCCGCcgCCA-GUG----------ACGGaa----CUGGA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 35473 | 0.71 | 0.170941 |
Target: 5'- uGUCGGUGGCGGcCAgcGCCggGGCCa -3' miRNA: 3'- -CGGCCGCCGCCaGUgaCGGaaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 35160 | 0.66 | 0.342082 |
Target: 5'- aGCCGGCuGGaggacGUCACUGUCggccaGGCCg -3' miRNA: 3'- -CGGCCG-CCgc---CAGUGACGGaa---CUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 31636 | 0.97 | 0.001751 |
Target: 5'- gGCCGGCGGCGG-UACUGCCUUGACCUc -3' miRNA: 3'- -CGGCCGCCGCCaGUGACGGAACUGGA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 30791 | 0.8 | 0.031868 |
Target: 5'- cGCCuGGCaGCGGUCGCgGCCUcGACCUg -3' miRNA: 3'- -CGG-CCGcCGCCAGUGaCGGAaCUGGA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 28293 | 0.71 | 0.153314 |
Target: 5'- aCCGGCGcuGCGGgaGCUGCCggGGCCg -3' miRNA: 3'- cGGCCGC--CGCCagUGACGGaaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 28234 | 0.66 | 0.333956 |
Target: 5'- cGCCGGCcgaGGCGGaUACaGCCgaggcgccGACCg -3' miRNA: 3'- -CGGCCG---CCGCCaGUGaCGGaa------CUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 27578 | 0.66 | 0.342082 |
Target: 5'- gGUCGGCGuCGGcCuucuuCUgcGCCUUGACCa -3' miRNA: 3'- -CGGCCGCcGCCaGu----GA--CGGAACUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 26312 | 0.66 | 0.36731 |
Target: 5'- cGCCauGGCGGC-GUCACUcGCCagGGCg- -3' miRNA: 3'- -CGG--CCGCCGcCAGUGA-CGGaaCUGga -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 26181 | 0.7 | 0.190357 |
Target: 5'- gGCgCGGCGGUGGcCGCUGCgggcgUGGCgCUg -3' miRNA: 3'- -CG-GCCGCCGCCaGUGACGga---ACUG-GA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 26089 | 0.67 | 0.295461 |
Target: 5'- cCCGaGgGGCGGaCGCUGCCgcagcgucGGCCa -3' miRNA: 3'- cGGC-CgCCGCCaGUGACGGaa------CUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 25898 | 0.71 | 0.153314 |
Target: 5'- uGCCGGCGcagaucaccgacGUGGUCACcaGCCU-GGCCa -3' miRNA: 3'- -CGGCCGC------------CGCCAGUGa-CGGAaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 25697 | 0.69 | 0.216757 |
Target: 5'- uGCCGGUcgaacucGGCGGUgGCUuucgccgcaGCCUcGGCCg -3' miRNA: 3'- -CGGCCG-------CCGCCAgUGA---------CGGAaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 24099 | 0.66 | 0.323605 |
Target: 5'- -aCGGUGGCGGUCuugccgauagggaaGCUGCgUaccagggcuuucUGGCCg -3' miRNA: 3'- cgGCCGCCGCCAG--------------UGACGgA------------ACUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 21074 | 0.74 | 0.090216 |
Target: 5'- gGCCGGUGGCGGU-GCUGCCgc--CCa -3' miRNA: 3'- -CGGCCGCCGCCAgUGACGGaacuGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 20945 | 0.68 | 0.260509 |
Target: 5'- gGUCGGUggucagGGCGGUCAUagcGCCUccuuacUGGCCUu -3' miRNA: 3'- -CGGCCG------CCGCCAGUGa--CGGA------ACUGGA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 20435 | 0.76 | 0.064014 |
Target: 5'- gGCCGGCuucagGGCGGUCAggGCCgcaaUGGCCUc -3' miRNA: 3'- -CGGCCG-----CCGCCAGUgaCGGa---ACUGGA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 20082 | 0.66 | 0.333956 |
Target: 5'- gGCCGGCaGCGGgCGCggGC--UGGCCg -3' miRNA: 3'- -CGGCCGcCGCCaGUGa-CGgaACUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 17406 | 0.67 | 0.295461 |
Target: 5'- aGCCGGC-GCGG--GCUGCCgagcugggcGACCUc -3' miRNA: 3'- -CGGCCGcCGCCagUGACGGaa-------CUGGA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 16703 | 0.66 | 0.333956 |
Target: 5'- gGCUGGCaGCGGgauucccaGCUGCUccuggacccagUUGACCg -3' miRNA: 3'- -CGGCCGcCGCCag------UGACGG-----------AACUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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