Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23061 | 5' | -48.8 | NC_005178.1 | + | 21984 | 0.66 | 0.90289 |
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Target: 5'- -cGCGAGC-UUCG-GGUUGgu-GAGCa -3' miRNA: 3'- uuCGCUCGaAAGCgCCGACaauUUUG- -5' |
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| 23061 | 5' | -48.8 | NC_005178.1 | + | 16046 | 0.67 | 0.842989 |
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Target: 5'- uAGCGGGCUguucCGCGuGCUGgccuGGCc -3' miRNA: 3'- uUCGCUCGAaa--GCGC-CGACaauuUUG- -5' |
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| 23061 | 5' | -48.8 | NC_005178.1 | + | 1780 | 0.68 | 0.823304 |
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Target: 5'- cGGCGAGCagugcaUCGCGGCcGU--AAGCc -3' miRNA: 3'- uUCGCUCGaa----AGCGCCGaCAauUUUG- -5' |
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| 23061 | 5' | -48.8 | NC_005178.1 | + | 4244 | 0.7 | 0.688729 |
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Target: 5'- gAAGCuGGCauguagUCGCGGCUGUUGc--- -3' miRNA: 3'- -UUCGcUCGaa----AGCGCCGACAAUuuug -5' |
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| 23061 | 5' | -48.8 | NC_005178.1 | + | 8651 | 0.72 | 0.592037 |
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Target: 5'- cGGCGAGCg--CGUGGUgcaGUUGGGGCc -3' miRNA: 3'- uUCGCUCGaaaGCGCCGa--CAAUUUUG- -5' |
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| 23061 | 5' | -48.8 | NC_005178.1 | + | 32450 | 1.08 | 0.00262 |
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Target: 5'- cAAGCGAGCUUUCGCGGCUGUUAAAACu -3' miRNA: 3'- -UUCGCUCGAAAGCGCCGACAAUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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