Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23072 | 5' | -57.3 | NC_005178.1 | + | 1423 | 1.13 | 0.000216 |
Target: 5'- aUCUUCGAUGUUCCGCAGCCGGCCCGCg -3' miRNA: 3'- -AGAAGCUACAAGGCGUCGGCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 11775 | 0.78 | 0.087197 |
Target: 5'- --aUgGggGUUCCGCcgcaGGCCGGCCUGCa -3' miRNA: 3'- agaAgCuaCAAGGCG----UCGGCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 16048 | 0.71 | 0.270363 |
Target: 5'- ---gCGGgcUGUUCCGCGuGCUGGCCUGg -3' miRNA: 3'- agaaGCU--ACAAGGCGU-CGGCCGGGCg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 7098 | 0.71 | 0.270363 |
Target: 5'- cUCggUCGA-GUgcagcgaguaccUCCaGCGGCCGGCgCCGCu -3' miRNA: 3'- -AGa-AGCUaCA------------AGG-CGUCGGCCG-GGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 25976 | 0.7 | 0.284561 |
Target: 5'- aUCUUCGG-GUUgUGCgggAGCUGGCCCuGCc -3' miRNA: 3'- -AGAAGCUaCAAgGCG---UCGGCCGGG-CG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 31961 | 0.7 | 0.291878 |
Target: 5'- --gUCGgcGUUCCGUAGCCuGGUCUauGCa -3' miRNA: 3'- agaAGCuaCAAGGCGUCGG-CCGGG--CG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 11659 | 0.7 | 0.306952 |
Target: 5'- --gUCGGUGaUCUGC-GCCGGCaacugCCGCa -3' miRNA: 3'- agaAGCUACaAGGCGuCGGCCG-----GGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 28319 | 0.69 | 0.322615 |
Target: 5'- aUCUUCGAUGcUUCggagucgugcaGCGGCgGGCCUuGCa -3' miRNA: 3'- -AGAAGCUAC-AAGg----------CGUCGgCCGGG-CG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 33914 | 0.69 | 0.347212 |
Target: 5'- gCggCGAUGcUCgGCGGCCGGUgCCGg -3' miRNA: 3'- aGaaGCUACaAGgCGUCGGCCG-GGCg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 2173 | 0.69 | 0.347212 |
Target: 5'- cUCgcUGGUGUUCgucaGCAGCCGGgCCUGg -3' miRNA: 3'- -AGaaGCUACAAGg---CGUCGGCC-GGGCg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 9035 | 0.68 | 0.364341 |
Target: 5'- gCUUCGAccUGgagcaaugCCGC-GCCGcGCUCGCg -3' miRNA: 3'- aGAAGCU--ACaa------GGCGuCGGC-CGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 6732 | 0.68 | 0.364341 |
Target: 5'- ---cUGAUGcgCCGCGuagacGCCGGCCCa- -3' miRNA: 3'- agaaGCUACaaGGCGU-----CGGCCGGGcg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 7551 | 0.68 | 0.381146 |
Target: 5'- cUUUCGAcgaauacggguUGUUCUGCAccgaguuGCCGGgCUGCg -3' miRNA: 3'- aGAAGCU-----------ACAAGGCGU-------CGGCCgGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 11926 | 0.68 | 0.382045 |
Target: 5'- cUCggCGGUGgcuuucgCCGCAGCCucGG-CCGCu -3' miRNA: 3'- -AGaaGCUACaa-----GGCGUCGG--CCgGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 8820 | 0.68 | 0.39111 |
Target: 5'- ---aCGAUGUaauggucgCCGCccuuGCCGGCCCa- -3' miRNA: 3'- agaaGCUACAa-------GGCGu---CGGCCGGGcg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 11878 | 0.68 | 0.399387 |
Target: 5'- --gUCGAUGg-CCGCgagcaguuugcccAGCuCGGCCUGCu -3' miRNA: 3'- agaAGCUACaaGGCG-------------UCG-GCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 34146 | 0.67 | 0.41913 |
Target: 5'- ---cCGGgacUUCCGgGGaCUGGCCCGCa -3' miRNA: 3'- agaaGCUac-AAGGCgUC-GGCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 32090 | 0.67 | 0.41913 |
Target: 5'- ---aCGGUGaacucUUCGCGGuuGGCCCGg -3' miRNA: 3'- agaaGCUACa----AGGCGUCggCCGGGCg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 6902 | 0.67 | 0.41913 |
Target: 5'- gUCUUCGuccagcUGcucgCCGCugAGgUGGCCCGCa -3' miRNA: 3'- -AGAAGCu-----ACaa--GGCG--UCgGCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 12629 | 0.67 | 0.455309 |
Target: 5'- --aUCGAgcguaaggccgugcUGUacggcUCgGCGGCCGGCgCGCu -3' miRNA: 3'- agaAGCU--------------ACA-----AGgCGUCGGCCGgGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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