Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23072 | 5' | -57.3 | NC_005178.1 | + | 14295 | 0.67 | 0.45631 |
Target: 5'- gUCUUCGGcuccggcaagcgGaUgCGCGGCCuucgacacGGCCCGCa -3' miRNA: 3'- -AGAAGCUa-----------CaAgGCGUCGG--------CCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 25303 | 0.67 | 0.458316 |
Target: 5'- ---cCGAguaguuUGUUggUCGCuGUCGGCCCGCu -3' miRNA: 3'- agaaGCU------ACAA--GGCGuCGGCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 25756 | 0.67 | 0.458316 |
Target: 5'- ----gGGUGUccuggUCCauguGCAgGCCGGCCUGCg -3' miRNA: 3'- agaagCUACA-----AGG----CGU-CGGCCGGGCG- -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 27225 | 0.67 | 0.468416 |
Target: 5'- gCUUCGccccUCCGCGcCCGGCCCu- -3' miRNA: 3'- aGAAGCuacaAGGCGUcGGCCGGGcg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 3091 | 0.66 | 0.499375 |
Target: 5'- aUCUggUCGAUGUauuUCaCGauaAGCUGGCUCGg -3' miRNA: 3'- -AGA--AGCUACA---AG-GCg--UCGGCCGGGCg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 16321 | 0.66 | 0.499375 |
Target: 5'- ---cCGAUGa--UGCGGCCGGCCgGg -3' miRNA: 3'- agaaGCUACaagGCGUCGGCCGGgCg -5' |
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23072 | 5' | -57.3 | NC_005178.1 | + | 35773 | 0.66 | 0.52051 |
Target: 5'- --cUUGA-GUUCaCGUgccuGCCGGCUCGCu -3' miRNA: 3'- agaAGCUaCAAG-GCGu---CGGCCGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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