Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23072 | 5' | -57.3 | NC_005178.1 | + | 11926 | 0.68 | 0.382045 |
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Target: 5'- cUCggCGGUGgcuuucgCCGCAGCCucGG-CCGCu -3' miRNA: 3'- -AGaaGCUACaa-----GGCGUCGG--CCgGGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 7551 | 0.68 | 0.381146 |
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Target: 5'- cUUUCGAcgaauacggguUGUUCUGCAccgaguuGCCGGgCUGCg -3' miRNA: 3'- aGAAGCU-----------ACAAGGCGU-------CGGCCgGGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 6732 | 0.68 | 0.364341 |
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Target: 5'- ---cUGAUGcgCCGCGuagacGCCGGCCCa- -3' miRNA: 3'- agaaGCUACaaGGCGU-----CGGCCGGGcg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 2173 | 0.69 | 0.347212 |
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Target: 5'- cUCgcUGGUGUUCgucaGCAGCCGGgCCUGg -3' miRNA: 3'- -AGaaGCUACAAGg---CGUCGGCC-GGGCg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 28319 | 0.69 | 0.322615 |
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Target: 5'- aUCUUCGAUGcUUCggagucgugcaGCGGCgGGCCUuGCa -3' miRNA: 3'- -AGAAGCUAC-AAGg----------CGUCGgCCGGG-CG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 11659 | 0.7 | 0.306952 |
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Target: 5'- --gUCGGUGaUCUGC-GCCGGCaacugCCGCa -3' miRNA: 3'- agaAGCUACaAGGCGuCGGCCG-----GGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 25976 | 0.7 | 0.284561 |
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Target: 5'- aUCUUCGG-GUUgUGCgggAGCUGGCCCuGCc -3' miRNA: 3'- -AGAAGCUaCAAgGCG---UCGGCCGGG-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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