Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23072 | 5' | -57.3 | NC_005178.1 | + | 27225 | 0.67 | 0.468416 |
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Target: 5'- gCUUCGccccUCCGCGcCCGGCCCu- -3' miRNA: 3'- aGAAGCuacaAGGCGUcGGCCGGGcg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 28319 | 0.69 | 0.322615 |
|
Target: 5'- aUCUUCGAUGcUUCggagucgugcaGCGGCgGGCCUuGCa -3' miRNA: 3'- -AGAAGCUAC-AAGg----------CGUCGgCCGGG-CG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 31961 | 0.7 | 0.291878 |
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Target: 5'- --gUCGgcGUUCCGUAGCCuGGUCUauGCa -3' miRNA: 3'- agaAGCuaCAAGGCGUCGG-CCGGG--CG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 32090 | 0.67 | 0.41913 |
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Target: 5'- ---aCGGUGaacucUUCGCGGuuGGCCCGg -3' miRNA: 3'- agaaGCUACa----AGGCGUCggCCGGGCg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 33914 | 0.69 | 0.347212 |
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Target: 5'- gCggCGAUGcUCgGCGGCCGGUgCCGg -3' miRNA: 3'- aGaaGCUACaAGgCGUCGGCCG-GGCg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 34146 | 0.67 | 0.41913 |
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Target: 5'- ---cCGGgacUUCCGgGGaCUGGCCCGCa -3' miRNA: 3'- agaaGCUac-AAGGCgUC-GGCCGGGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 35773 | 0.66 | 0.52051 |
|
Target: 5'- --cUUGA-GUUCaCGUgccuGCCGGCUCGCu -3' miRNA: 3'- agaAGCUaCAAG-GCGu---CGGCCGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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