Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23072 | 5' | -57.3 | NC_005178.1 | + | 7551 | 0.68 | 0.381146 |
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Target: 5'- cUUUCGAcgaauacggguUGUUCUGCAccgaguuGCCGGgCUGCg -3' miRNA: 3'- aGAAGCU-----------ACAAGGCGU-------CGGCCgGGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 7098 | 0.71 | 0.270363 |
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Target: 5'- cUCggUCGA-GUgcagcgaguaccUCCaGCGGCCGGCgCCGCu -3' miRNA: 3'- -AGa-AGCUaCA------------AGG-CGUCGGCCG-GGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 6902 | 0.67 | 0.41913 |
|
Target: 5'- gUCUUCGuccagcUGcucgCCGCugAGgUGGCCCGCa -3' miRNA: 3'- -AGAAGCu-----ACaa--GGCG--UCgGCCGGGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 6732 | 0.68 | 0.364341 |
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Target: 5'- ---cUGAUGcgCCGCGuagacGCCGGCCCa- -3' miRNA: 3'- agaaGCUACaaGGCGU-----CGGCCGGGcg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 3091 | 0.66 | 0.499375 |
|
Target: 5'- aUCUggUCGAUGUauuUCaCGauaAGCUGGCUCGg -3' miRNA: 3'- -AGA--AGCUACA---AG-GCg--UCGGCCGGGCg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 2173 | 0.69 | 0.347212 |
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Target: 5'- cUCgcUGGUGUUCgucaGCAGCCGGgCCUGg -3' miRNA: 3'- -AGaaGCUACAAGg---CGUCGGCC-GGGCg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 1423 | 1.13 | 0.000216 |
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Target: 5'- aUCUUCGAUGUUCCGCAGCCGGCCCGCg -3' miRNA: 3'- -AGAAGCUACAAGGCGUCGGCCGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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