Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23072 | 5' | -57.3 | NC_005178.1 | + | 11659 | 0.7 | 0.306952 |
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Target: 5'- --gUCGGUGaUCUGC-GCCGGCaacugCCGCa -3' miRNA: 3'- agaAGCUACaAGGCGuCGGCCG-----GGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 31961 | 0.7 | 0.291878 |
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Target: 5'- --gUCGgcGUUCCGUAGCCuGGUCUauGCa -3' miRNA: 3'- agaAGCuaCAAGGCGUCGG-CCGGG--CG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 25976 | 0.7 | 0.284561 |
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Target: 5'- aUCUUCGG-GUUgUGCgggAGCUGGCCCuGCc -3' miRNA: 3'- -AGAAGCUaCAAgGCG---UCGGCCGGG-CG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 7098 | 0.71 | 0.270363 |
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Target: 5'- cUCggUCGA-GUgcagcgaguaccUCCaGCGGCCGGCgCCGCu -3' miRNA: 3'- -AGa-AGCUaCA------------AGG-CGUCGGCCG-GGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 16048 | 0.71 | 0.270363 |
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Target: 5'- ---gCGGgcUGUUCCGCGuGCUGGCCUGg -3' miRNA: 3'- agaaGCU--ACAAGGCGU-CGGCCGGGCg -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 11775 | 0.78 | 0.087197 |
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Target: 5'- --aUgGggGUUCCGCcgcaGGCCGGCCUGCa -3' miRNA: 3'- agaAgCuaCAAGGCG----UCGGCCGGGCG- -5' |
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| 23072 | 5' | -57.3 | NC_005178.1 | + | 1423 | 1.13 | 0.000216 |
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Target: 5'- aUCUUCGAUGUUCCGCAGCCGGCCCGCg -3' miRNA: 3'- -AGAAGCUACAAGGCGUCGGCCGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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