Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23073 | 3' | -55 | NC_005178.1 | + | 22410 | 0.66 | 0.642246 |
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Target: 5'- aGCUUGU-CGGCG-UGugGUGugaucgcucgGGCAGGu -3' miRNA: 3'- -CGGACAgGCCGCuACugCAC----------UCGUUC- -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 28948 | 0.66 | 0.619637 |
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Target: 5'- gGCCggagacuUCCGGCGAgcGCGUGGuGCAGu -3' miRNA: 3'- -CGGac-----AGGCCGCUacUGCACU-CGUUc -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 36787 | 0.66 | 0.619637 |
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Target: 5'- uGCUUGauagCCGGCGAgcauUGGCGa-AGCAGGu -3' miRNA: 3'- -CGGACa---GGCCGCU----ACUGCacUCGUUC- -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 8601 | 0.66 | 0.597075 |
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Target: 5'- aCCUGgcgCaCGGCGAUGGcCGUGccggacGCGGGg -3' miRNA: 3'- cGGACa--G-GCCGCUACU-GCACu-----CGUUC- -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 21732 | 0.67 | 0.574637 |
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Target: 5'- cCCUGU-CGGUGAUGccuCGgUGAGCAAc -3' miRNA: 3'- cGGACAgGCCGCUACu--GC-ACUCGUUc -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 27742 | 0.67 | 0.541375 |
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Target: 5'- cGCCUGgacCUGGCGAgccaguacggUGACGgcucGCGAGg -3' miRNA: 3'- -CGGACa--GGCCGCU----------ACUGCacu-CGUUC- -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 20444 | 0.68 | 0.530426 |
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Target: 5'- gGCCUGgcCCaGGaCGcgGACGUcGAGCAGa -3' miRNA: 3'- -CGGACa-GG-CC-GCuaCUGCA-CUCGUUc -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 3235 | 0.69 | 0.46668 |
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Target: 5'- aGCCgcgagGUCgGGCGcAUGG-GUGAGCAu- -3' miRNA: 3'- -CGGa----CAGgCCGC-UACUgCACUCGUuc -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 33823 | 0.71 | 0.335568 |
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Target: 5'- -gCUGUUCGGUGGUGACaaGGGCGAa -3' miRNA: 3'- cgGACAGGCCGCUACUGcaCUCGUUc -5' |
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| 23073 | 3' | -55 | NC_005178.1 | + | 1555 | 1.12 | 0.000445 |
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Target: 5'- cGCCUGUCCGGCGAUGACGUGAGCAAGa -3' miRNA: 3'- -CGGACAGGCCGCUACUGCACUCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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