Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23104 | 5' | -55.6 | NC_005178.1 | + | 28141 | 0.66 | 0.600962 |
Target: 5'- aUGGCGGCGCccagGGgcuGACGGUCg- -3' miRNA: 3'- aGCCGCCGUGuugaCUu--CUGCCAGgu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 9911 | 0.66 | 0.589727 |
Target: 5'- gCGGCGGCACGuugaccCUGGAG--GG-CCAg -3' miRNA: 3'- aGCCGCCGUGUu-----GACUUCugCCaGGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 29675 | 0.66 | 0.578529 |
Target: 5'- aUCGGCGccgcGCACAgucACggacgGggGACGGaugcgCCAc -3' miRNA: 3'- -AGCCGC----CGUGU---UGa----CuuCUGCCa----GGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 28710 | 0.66 | 0.612225 |
Target: 5'- aUGGCGGCcgACAugUGGgccggcaaGGGCGGcgaCCAu -3' miRNA: 3'- aGCCGCCG--UGUugACU--------UCUGCCa--GGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 23856 | 0.66 | 0.589727 |
Target: 5'- aCGGCGGCAUc----AAGGCGG-CCAc -3' miRNA: 3'- aGCCGCCGUGuugacUUCUGCCaGGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 20008 | 0.66 | 0.600962 |
Target: 5'- uUCGGCGGCAUgauguGCUGccAGAUcgGGUCg- -3' miRNA: 3'- -AGCCGCCGUGu----UGACu-UCUG--CCAGgu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 26266 | 0.66 | 0.612225 |
Target: 5'- -gGGCGGacucgaACAGCUcGGGGCGGUUgGa -3' miRNA: 3'- agCCGCCg-----UGUUGAcUUCUGCCAGgU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 21347 | 0.66 | 0.600962 |
Target: 5'- gCuGCGGCAUGGCcGAGGcCGGUCa- -3' miRNA: 3'- aGcCGCCGUGUUGaCUUCuGCCAGgu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 15017 | 0.66 | 0.600962 |
Target: 5'- -gGGCGGUcccuCuGCUGggGugGGUUa- -3' miRNA: 3'- agCCGCCGu---GuUGACuuCugCCAGgu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 5314 | 0.66 | 0.578529 |
Target: 5'- aUCGGuCGGCuCGACc--AGGCGGcCCAg -3' miRNA: 3'- -AGCC-GCCGuGUUGacuUCUGCCaGGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 4059 | 0.66 | 0.567378 |
Target: 5'- cCGGUGGCAguAgccCUGucGAgUGGUCCAg -3' miRNA: 3'- aGCCGCCGUguU---GACuuCU-GCCAGGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 31231 | 0.66 | 0.600962 |
Target: 5'- -aGGCGGUcccACGACUGAcaaggAGGCacccaGUCCAu -3' miRNA: 3'- agCCGCCG---UGUUGACU-----UCUGc----CAGGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 10278 | 0.67 | 0.50191 |
Target: 5'- cUCGGCguuccugaaaucGGUuuGCAACUGGuccAGGCGGUUCu -3' miRNA: 3'- -AGCCG------------CCG--UGUUGACU---UCUGCCAGGu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 22308 | 0.67 | 0.491307 |
Target: 5'- gCGGCcgggaaguGGCGCAGCUGuugcuccAGAaCGGUCUg -3' miRNA: 3'- aGCCG--------CCGUGUUGACu------UCU-GCCAGGu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 20498 | 0.67 | 0.50191 |
Target: 5'- cCGGCcGCAUGGC-GAAGAUGGcCCGc -3' miRNA: 3'- aGCCGcCGUGUUGaCUUCUGCCaGGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 35163 | 0.67 | 0.512613 |
Target: 5'- cUCGGUGGCAUGGC-GGAGAUcGUCgAg -3' miRNA: 3'- -AGCCGCCGUGUUGaCUUCUGcCAGgU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 12655 | 0.67 | 0.53429 |
Target: 5'- cUCGGCGGC-CGGCgcgcuGGCGGguggCCu -3' miRNA: 3'- -AGCCGCCGuGUUGacuu-CUGCCa---GGu -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 28210 | 0.67 | 0.53429 |
Target: 5'- cUGGCGGCACGcucCUGA--GCGGcgCCGc -3' miRNA: 3'- aGCCGCCGUGUu--GACUucUGCCa-GGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 10298 | 0.67 | 0.512613 |
Target: 5'- cUCGGCGGCACGuccu--GGCGGauagCCGa -3' miRNA: 3'- -AGCCGCCGUGUugacuuCUGCCa---GGU- -5' |
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23104 | 5' | -55.6 | NC_005178.1 | + | 10326 | 0.67 | 0.54525 |
Target: 5'- cUCGGUGacGCugGACUGGucgaggggcguAGACGGcCCu -3' miRNA: 3'- -AGCCGC--CGugUUGACU-----------UCUGCCaGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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