miRNA: 3'- aaGgGGGUCCCGCgGCCGuUCCCGgc -5'
miRNA: 3'- aaggGGGUCCCGCgGCCGuUCCCGgc -5'
miRNA: 3'- aaGGgGGUCCCGCGGCCG-UUCC--------CGGC- -5'
miRNA: 3'- -AAGGGGGUCCCGCGGCCGUUCCCGGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23109 | 3' | -66.9 | NC_005178.1 | + | 23444 | 0.71 | 0.053014 |
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Target: 5'- -aCgCCCAGGGCGaCGGCcAGGGCg- -3' miRNA: 3'- aaGgGGGUCCCGCgGCCGuUCCCGgc -5' |
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| 23109 | 3' | -66.9 | NC_005178.1 | + | 26402 | 0.72 | 0.051544 |
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Target: 5'- ---gCCCAGGGCGgCGGCcAGGGCg- -3' miRNA: 3'- aaggGGGUCCCGCgGCCGuUCCCGgc -5' |
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| 23109 | 3' | -66.9 | NC_005178.1 | + | 9368 | 0.73 | 0.036727 |
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Target: 5'- -gUCgCCAGGGCGCCGGCcGAGGcggauacaGCCGa -3' miRNA: 3'- aaGGgGGUCCCGCGGCCG-UUCC--------CGGC- -5' |
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| 23109 | 3' | -66.9 | NC_005178.1 | + | 14219 | 1.04 | 9.2e-05 |
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Target: 5'- cUUCCCCCAGGGCGCCGGCAAGGGCCGc -3' miRNA: 3'- -AAGGGGGUCCCGCGGCCGUUCCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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