Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23124 | 5' | -56.7 | NC_005178.1 | + | 28510 | 0.72 | 0.229803 |
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Target: 5'- -aGCGCGGcGCggcauUGCUcCAGGucGAAGCCGGc -3' miRNA: 3'- gaCGCGCC-CG-----ACGA-GUUC--UUUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 30633 | 0.66 | 0.522196 |
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Target: 5'- gCUGCuGCGGGCcacCUCAGcggcGAGcAGCUGGa -3' miRNA: 3'- -GACG-CGCCCGac-GAGUU----CUU-UCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 31717 | 0.67 | 0.479788 |
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Target: 5'- uCUGCGCGGGUgucGUUCcuGAuaaugGAGCCc- -3' miRNA: 3'- -GACGCGCCCGa--CGAGuuCU-----UUCGGcc -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 32136 | 0.68 | 0.413788 |
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Target: 5'- -gGCGgGGGCucuugcccccgaccaUGCagAGGuGAGCCGGg -3' miRNA: 3'- gaCGCgCCCG---------------ACGagUUCuUUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 33012 | 0.7 | 0.313997 |
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Target: 5'- aCUGgGCGGcGUccgGCUCGauGGGcAGGCCGGa -3' miRNA: 3'- -GACgCGCC-CGa--CGAGU--UCU-UUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 33196 | 0.75 | 0.143801 |
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Target: 5'- gCUGCGCgaccGGaGUUGCUCGGGGgugaucgugccgAGGCCGGg -3' miRNA: 3'- -GACGCG----CC-CGACGAGUUCU------------UUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 33345 | 0.66 | 0.500794 |
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Target: 5'- uCU-CGCGGGCacGCUCGAcauacGAAGCUGGc -3' miRNA: 3'- -GAcGCGCCCGa-CGAGUUc----UUUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 33698 | 0.69 | 0.330111 |
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Target: 5'- aUGCGCGGcaaGCUGCUguGc--GGCCGGu -3' miRNA: 3'- gACGCGCC---CGACGAguUcuuUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 35802 | 0.68 | 0.419557 |
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Target: 5'- cCU-CGCGGGUccGCUCAAGgcGGCCc- -3' miRNA: 3'- -GAcGCGCCCGa-CGAGUUCuuUCGGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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